Comparative metabolomics analysis of unique yellow hawthorn(Crataegus pinnatifida)and red-skinned cultivars reveals a different polyphenol biosynthesis flux and antioxidative and antidiabetic potential  

作　　者：Yilong Liu Wenwen You Ruoqi Wang Tao Zeng Xiaoyong Zhao Yunlin Cao Wenna Zhang Zongxing Nie Xiaochun Yang Xian Li 刘意隆;尤文雯;王若绮;曾涛;赵晓勇;曹运琳;张文娜;聂宗省;杨晓春;李鲜(Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology,Zhejiang University,Hangzhou,China;Shandong(Linyi Institute of Modern Agriculture,Zhejiang University,Linyi,China;Center for Drug Safety Evaluation and Research,College of Pharmaceutical Sciences,Zhejiang University,Hangzhou,China;Damenshan Hawthorn Research Institute in Fei County,Linyi,China)

机构地区：[1]Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology,Zhejiang University,Hangzhou,China [2]Shandong(Linyi)Institute of Modern Agriculture,Zhejiang University,Linyi,China [3]Center for Drug Safety Evaluation and Research,College of Pharmaceutical Sciences,Zhejiang University,Hangzhou,China [4]Damenshan Hawthorn Research Institute in Fei County,Linyi,China

出　　处：《Food Quality and Safety》2024年第3期520-533,共14页食品品质与安全研究（英文版）

基　　金：supported by the Key Research and Development Program of Zhejiang Province(2021C02001);the Natural Science Foundation of Shandong Province(No.ZR2023QC228);the Serving Local Economic Development Project of Shandong(Linyi)Institute of Modern Agriculture,Zhejiang University(No.ZDNY-2021-FWLY01004);the Fundamental Research Funds for the Central Universities(No.226-2022-00215),China.

摘　　要：Objectives:This paper aims to investigate the polyphenol metabolite variation and bioactivities of different-colored hawthorn berries(Crataegus pinnatifida).Materials and Methods:Comparative metabolomics analysis between peel and flesh of yellow hawthorn'Jinruyi'(JRY)and red-skinned ones was carried out by UPLC-MS/MS.Antioxidant activities andα-glucosidase inhibition capacity were also tested among different colored hawthorn samples.Results:A total of 453 polyphenols was characterized,among which phenolic acids and flavonoids were abundant,and were closely relevant to the antioxidant capacity of hawthorn fruits.Polyphenol profile showed accession-specific accumulation in peel or flesh of different colored hawthorn.The unique yellow hawthorn was found to have few anthocyanins but showed enhanced flux to synthesize flavones and flavonols,especially flavone C-glycosides and acylated flavonol glycosides.The specific acylation decoration included acetylation,p-coumaroylation,and malonylation.In addition,yellow hawthorn showed excellentα-glucosidase inhibitory effect,which might be associated with the high concentration of 8 polyphenols including 5 phenolic acids,2 flavone C-glycosides,and an acylated flavonol glycoside,namely,quercetin-7-O-(6ʹʹ-malonyl)glucoside.Such acylated flavonol showed the strongest correlation with the inhibition effect of hawthorn fruits onα-glucosidase,and was predicted to have the lowest binding energy with the enzyme according to molecular docking analysis,indicating its great potential as a strongα-glucosidase inhibitor and an important antidiabetic ingredient in yellow hawthorn.Conclusions:The acylated flavonol glycosides and C-glycosyl flavones might be chemotaxonomic markers differentiating varieties and bioactivities of yellow hawthorn from the traditional red-skinned ones.These findings complement the existing knowledge on the metabolite composition and nutritional properties of hawthorn fruits.

关 键 词：Hawthorn fruit comparative metabolomics analysis antioxidant activity α-glucosidase inhibitory effect polyphenol biosynthesis 

分 类 号：TS201.4[轻工技术与工程—食品科学]