Propofol suppressed cell proliferation through inhibition of SREBP1c-mediated De novo lipogenesis in colorectal cancer cells  

作　　者：YAJUN CAO SHUANG YIN YIDAN FANG JIEXIAN ZHOU YOUTAN LIU 

机构地区：[1]Department of Anesthesiology,Shenzhen Hospital,Southern Medical University,Shenzhen,518000,China [2]The Third School of Clinical Medicine,Southern Medical University,Guangzhou,510630,China [3]Department of Anesthesia,Zhuhai Center for Maternal and Child Health Care,Zhuhai,519000,China [4]Department of Anesthesia,Zhuhai Doumen Maternal and Child Health Care Hospital,Zhuhai,519000,China

出　　处：《BIOCELL》2024年第12期1773-1780,共8页生物细胞（英文）

基　　金：supported by Zhuhai Science and Technology Plan Project in the Field of Social Development(2320004000157);National Natural Science Foundation of China(82072215,82272219);Shenzhen Science and Technology Program(JCYJ20210324134602006);Natural Science Foundation of Guangdong Province(2214050001873).

摘　　要：Background: De novo lipogenesis (DNL) is a critical event for the development of tumors, in the present work,we revealed the role of propofol in colorectal cancer (CRC) cell proliferation. Methods: Western blotting (WB), Real-timePCR, and luciferase combined with chromatin immunoprecipitation (ChIP) were used to identify the mechanismunderlying propofol-modulated cell proliferation in CRC cells. Results: Herein, we showed that propofol suppressedcell proliferation, which was attributed to the inhibition of DNL characterized by reduced fatty acid synthase (FASN),acetyl-coA carboxylase alpha (ACCA), and stearoyl-coA desaturase-1 (SCD1) expression. Mechanically, propofolstimulation decreased sterol regulatory element-binding proteins-1c (SREBP-1c) mature and nuclear translocation,which further decreased SCD1 transactivation confirmed by luciferase and ChIP analysis, while no significantdifference in total SREBP1c was observed. What’s more, supplementation of Monounsaturated fatty acid (MuFA)could reverse the inhibitory effect of propofol on cell proliferation. Conclusion: Taken together, these resultssuggested propofol modulated cell proliferation is dependent on SREBP1c-mediated DNL.

关 键 词：PROPOFOL SREBP1c De novo lipogenesis Cell proliferation Colorectal cancer cells 

分 类 号：R735.35[医药卫生—肿瘤]