不同地域引栽樟叶越橘嫩叶中有效成分含量及其抗氧化活性差异分析  

Analysis of the Differences in Active Ingredients Content and Antioxidant Activity in Tender Leaves of Vaccinium dunalianum Introduced From Different Regions

作　　者：姚传慧 谢东 赵平[1] 徐俊明[2] 何远平 杨晓琴[1] YAO Chuanhui;XIE Dong;ZHAO Ping;XU Junming;HE Yuanping;YANG Xiaoqin(Key Laboratory of Efficient Utilization of Forestry Biomass Resources in Southwest China,Key Laboratory of National Forestry and Grassland Administration,Southwest Forestry University,Kunming 650224,China;Institute of Chemical Industry of Forest Products,Chinese Academy of Forestry,Nanjing 210042,China;Kunming Yuanfang Biological Products Co.,Ltd,Kunming 650499,China)

机构地区：[1]西南林业大学西南地区林业生物质资源高效利用国家林业和草原局重点实验室,云南昆明650224 [2]中国林业科学研究院林产化学工业研究所,江苏南京210042 [3]昆明远方生物制品有限公司,云南昆明650499

出　　处：《林产化学与工业》2024年第6期38-46,共9页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(22267018);云南省中青年学术和技术带头人后备人才项目(202205AC160049);昆明远方生物制品有限公司赵平专家基层科研工作站(无编号)。

摘　　要：以不同地域引栽的樟叶越橘(Vaccinium dunalianum Wight)嫩叶为研究对象,采用电感耦合等离子体原子发射光谱(ICP-AES)仪分析干燥嫩叶中的矿质元素,采用高效液相色谱(HPLC)分析嫩叶醇提液中的主要成分及含量,采用福林酚-标准曲线法分析嫩叶醇提液中的总多酚含量,通过DPPH·、·ABTS+清除能力、Fe^(3+)还原能力综合分析嫩叶醇提液的体外抗氧化活性,并通过Pearson法对嫩叶醇提液总多酚含量和抗氧化活性进行相关性分析。研究结果表明:引栽自不同地域的越橘嫩叶中富含K和Ca元素,24种矿物质元素含量虽有差异但变化规律较为一致;醇提液中主要3大酚类含量依次为6'-O-咖啡酰熊果苷>β-熊果苷>绿原酸;总多酚质量分数为42.79~53.36 mg/g,对DPPH·和·ABTS+清除率的半数抑制质量浓度(IC 50)值分别为6.26~8.95 mg/L和8.17~14.35 mg/L,Fe^(3+)还原能力显著;相关性分析发现6'-O-咖啡酰熊果苷、总多酚含量和抗氧化活性存在显著正相关性,因此,樟叶越橘嫩叶中6'-O-咖啡酰熊果苷和总多酚的含量均可作为评判其作为雀嘴茶原料的重要品质指标。The research focused on tender leaves of Vaccinium dunalianum Wight from different regions.The mineral elements in dried tender leaves were analyzed using Inductively Coupled Plasma Atomic Emission Spectrometry(ICP-AES).High-performance liquid chromatography(HPLC)was employed to analyze the major components and their concentrations in the alcohol extract of tender leaves.The total polyphenol in the alcohol extract of tender leaves was determined using the Folin-Ciocalteu method.The antioxidant activity in the alcohol extract of tender leaves in vitro was assessed by the analysis of DPPH·and·ABTS+scavenging capabilities,as well as Fe^(3+)reduction capacity.The correlation between total polyphenol content and antioxidant activity of the alcohol extract of tender leaves was analyzed by Pearson method.The results showed that tender leaves of V.dunalianum from different regions were rich in potassium(K)and calcium(Ca).Although there were variations in mineral elements content,their changing patterns were relatively consistent.The three major phenolic compounds in the alcohol extract were in the order of 6'-O-caffeoyl arbutin>β-arbutin>chlorogenic acid.The total polyphenol content ranged from 42.79 to 53.36 mg/g,with semi-inhibitory mass concentration(IC 50)values for DPPH·and·ABTS+clearance ranged from 6.26 to 8.95 mg/L and 8.17 to 14.35 mg/L,respectively,indicating significant reduction capacity.Correlation analysis revealed that there was a significant positive correlation between 6'-O-caffeoyl arbutin,the total polyphenol content,as well as antioxidant activity.Therefore,the content of 6'-O-caffeoyl arbutin,and total polyphenols could serve as important quality indicators for evaluating tender leaves of V.dunalianum as raw materials for Quezui tea.

关 键 词：樟叶越橘 总多酚 6'-O-咖啡酰熊果苷 抗氧化活性 

分 类 号：TQ35[化学工程]