基于超高效液相色谱-质谱法脂质组学分析油莎草3个生长时期及组织脂类物质组成  

Lipidomics Analysis of the Lipid Composition of Three Tissues of Cyperus esculentus L.during Three Growth Stages Based on Ultra-high Performance Liquid Chromatography-Mass Spectrometry

作　　者：杨雅萱 卞苗苗 刘艳庆 刘军[1,2] 邢军 涂亦娴[1,2] YANG Yaxuan;BIAN Miaomiao;LIU Yanqing;LIU Jun;XING Jun;TU Yixian(College of Life Science and Technology,Xinjiang University,Ürümqi 830017,China;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Ürümqi 830017,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830017 [2]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,新疆乌鲁木齐830017

出　　处：《食品科学》2025年第3期146-161,共16页Food Science

基　　金：新疆维吾尔自治区自然科学基金资助项目(2020D01C018)。

摘　　要：为了深入探究油莎草不同生长时间不同组织的脂质组成、脂质动态变化,本研究基于超高效液相色谱-质谱法非靶向脂质组学对油莎草3个不同生长时期(70、93、120 d)地上茎、块茎、根中的脂类代谢物进行相对定量分析。结果显示,从油莎草上述组织中共鉴定出16 904种脂类代谢物,筛选出720个脂类差异代谢物,其中油莎草块茎差异脂类物质含量显著高于地上茎和根,油莎草在生长过程中积累的脂质主要为甘油脂且大部分以甘油三酯的形式存在,油莎草3种组织的脂肪酸(fatty acid,FA)中不饱和脂肪酸的相对含量较高,均超过57.3%,块茎中不饱和脂肪酸FA(18:1)和FA(18:2)的含量较高。本研究从脂质代谢层面分析了油莎草不同生长时期不同组织的脂质差异代谢物、脂质组成及变化,可为其在食品加工和产品开发等领域的应用提供基础数据和科学依据。In order to investigate changes in the lipid composition of different tissues of Cyperus esculentus L.at different growth stages,non-targeted lipidomics based on ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS)was employed for relative quantitative analysis of the lipid metabolites in the above-ground stems,tubers,and roots of C.esculentus L.after 70,93,and 120 days of growth.A total of 16904 lipid metabolites were identified and 720 differential lipid metabolites were found between the three tissues,the contents of differential lipid metabolites being significantly higher in the tubers than in the above-ground stems and roots.The main lipids accumulated in the growth process of C.esculentus L.were triacylglycerols(TAG).All three tissues had high proportions(higher than 57.3%)of unsaturated fatty acids relative to the total fatty acids(FAs),and the relative contents of unsaturated fatty acid FA(18:1)and FA(18:2)in the tubers were also high.This study provides basic data and a scientific basis for the application of C.esculentus L.in various fields such as food processing and product development.

关 键 词：油莎草 脂质组学 块茎 超高效液相色谱-质谱 脂肪酸 

分 类 号：TS222.1[轻工技术与工程—粮食、油脂及植物蛋白工程]