反义寡核苷酸抑制端粒酶活性对鼻咽癌细胞增殖的影响  

Effect of antisense oligodeoxynucleotide targeting against telomerase on proliferation of nasopharyngeal carcinoma cells

作　　者：曹前[1] 吴家廉[1] 骆文龙[1] 张利萍[2] 

机构地区：[1]重庆医科大学附属第二医院耳鼻咽喉科,400010 [2]重庆医科大学附属第二医院检验科,400010

出　　处：《重庆医学》2002年第12期1164-1166,共3页Chongqing medicine

摘　　要：目的　检测针对端粒酶的反义寡核苷酸 (AS ODN)对鼻咽癌HNE 1细胞株增殖的影响。方法　用PCR ELISA法检测细胞内端粒酶活性。采用MTT法及平板集落形成实验评价细胞增殖活性。结果　鼻咽癌细胞株HNE 1与 5 μmol/L ,2 0 μmol/L反义寡核苷酸共同孵育 1d ,对其细胞内端粒酶活性抑制率分别为 32 .9%、6 1.6 %。而同浓度正义寡核苷酸 (S ODN)对细胞端粒酶活性无抑制作用。 5 μmol/L、2 0 μmol/L反义寡核苷酸与HNE 1细胞共同孵育 2d,对细胞增殖的抑制率分别为34.4 % ,72 .9% ,同浓度正义寡核苷酸的抑制率为 14 .4 %、15 .6 %。 2 0 μmol/L反义寡核苷酸和 2 0 μmol/L正义寡核苷酸对细胞克隆形成能力的抑制率分别为 78.39%、2 3.37%。结论　该反义寡核苷酸可抑制鼻咽癌细胞端粒酶活性 ,此作用有序列特异性和浓度依赖性。该反义寡核苷酸明显抑制了鼻咽癌细胞HNE 1的增殖 ,此作用有剂量、时间依赖性和序列特异性。Objective To investigate the proliferation inhibition of AS ODN targeting against telomerase on HNE 1 cell strain in vitro. Methods Telomerase activity of HNE 1 cell strain was analysed by PCR ELISA technique. The proliferation activity of HNE 1 cell strain was detected by MTT colorimetric assay and clone formation test. Result HNE 1 cells were incubated with 5μmol/L,20μmol/L AS ODN for 1 day,the inhibition rate of telomerase activity was 32.9%,61.6% respectively.The S ODN did not inhibite telomerase activity of HNE 1 cell strain at the same concentration. HNE 1 cells were incubated with 5μmol/L,20μmol/L AS ODN for 2 days,the inhibition rate of cell proliferation was 34.4%,72.9% respectively,while it was 14.4%,15.6% to 5μmol/L,20μmol/L S ODN. 20μmol/L AS ODN inhibited 78.39% HNE 1 cells formating clones,while it was 23.37% to 20μmol/L S ODN. Conclusion The AS ODN inhibits telomerase activity of HNE 1 cells. This procedure is sequential specific. The AS ODN significantly inhibits the proliferation of HNE 1 cells,which shows a dose dependent and time dependent correlation and is sequential specific as compared with S ODN control group.

关 键 词：细胞增殖 鼻咽癌 反义寡核苷酸 端粒酶 PCR-ELISA法 

分 类 号：R739.63[医药卫生—肿瘤]