重组腺相关病毒介导人内皮抑素抑瘤作用的实验研究  

Research of the Anti-Tumor Effect of Human EndostatinMediated by Recombinant Adeno-Associated Virus

作　　者：路国秋[1] 查园园[1] 吴小兵[2] 杨建国[1] 张雪艳[1] 付明[1] 梁肖[1] 吴旻[1] 林晨[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所分子肿瘤学国家重点实验室,北京100021 [2]中国预防医学科学院病毒研究所病毒基因工程国家重点实验室,北京100052

出　　处：《癌症》2002年第12期1314-1318,共5页Chinese Journal of Cancer

基　　金：国家自然科学基金项目(No.39870839)

摘　　要：背景与目的:研究表明,内皮抑素能抑制肿瘤血管的生成,进而抑制肿瘤的生长和转移,然而有关内皮抑素治疗的研究报道却很少。本研究初步探讨了重组腺相关病毒介导内皮抑素对肿瘤生长和转移的作用。方法:用RT-PCR方法获得人内皮抑素基因,构建内皮抑素可分泌表达的通用型重组腺相关病毒(rAAV)载体,包装获得重组腺相关病毒rAAV-SS-Endostatin。以动物实验分析其抗肿瘤作用。结果:C57BL/6小鼠肌肉注射1011TUrAAC-SS-Endostatin一次,该重组病毒对小鼠黑色素瘤B16F10移植瘤生长的抑制率为57.1%,在实验肺转移模型中,对转移灶的抑制率为70.7%。结论:重组腺相关病毒介导的内皮抑素能有效抑制肿瘤的生长和转移。Background & Objective:It was reported that endostatin could inhibit tumor angiogenesis,then inhibit the growth and metastasis of tumor. However,there was few report about the treatment usage of endostatin. This study was designed to explore the effect of endostatin mediated with recombinant adeno associated virus(rAAV) on tumor growth and metastasis. Methods:To obtain the endostatin gene complete cDNA by RT PCR,and clone it into the plasmid pSNAV and package the recombinant rAAV SS endostatin;to analyze its anti tumor effect through animal experiments. Results:In the B16F10 melanoma tumor model,C57BL/6 mice were inoculated with 1011TU rAAV SS endostatin i.m. injection, which the inhibition rate was 57.1%;In the lung metastatic model,the inhibition rate of metastases was 70.7%. Conclusions:rAAV SS endostatin can effectively inhibit tumor growth and metastasis.

关 键 词：重组腺相关病毒 内皮抑素 肿瘤 基因治疗 实验研究 

分 类 号：R73-36[医药卫生—肿瘤]