卵磷脂的薄层扫描法测定  

Determination of the content of lecithin with thin-layer chromatographic scanner

作　　者：彭一鸣[1] 蒋笃孝[1] 刘慧琼[1] 

机构地区：[1]广州暨南大学化学系,广东广州510326

出　　处：《暨南大学学报（自然科学与医学版）》2002年第5期56-59,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国务院侨办重点学科基金项目(93A119)

摘　　要：　采用薄层扫描法测定卵磷脂质量的方法,以V(氯仿)∶V(无水乙醇)∶V(三乙胺)∶V(水)=10∶11 3∶11 7∶2 7为展开剂,溴百里酚蓝碱液为显色剂,日本岛津CS-930型薄层扫描仪定量扫描.测得精密度变异系数为2 45%,稳定性变异系数为0 364%,回收率在100 4%～102 2%范围内.该方法简捷方便,重现性,稳定性较好.The content of lecithin was determined by thin-layer chromatographic scanner. Chloroform-ethanol-triethylamine-water(10∶11.3∶11.7∶2.7) was used as developing solvent on an TLC silica plate. Bromothymol blue was used for coloration of the samples. The results showed that the repetition was 2.45% and the stability was 0.346%. The recovery rate was 100.4%～102.2%. It could be concluded that this method is a simple one with good repetition and stability. 

关 键 词：卵磷脂 薄层扫描法 展开齐 含量测定 显色剂 质量检测 

分 类 号：Q545.03[生物学—生物化学]