B细胞特异性激活蛋白在经典型霍奇金淋巴瘤中的表达及其意义  

Expression and significance of B-cell-specific activator protein of H/RS cell in classical Hodgkin′s Lymphoma

作　　者：周新华[1] 赵彤[1] 齐宗利[1] 柳刚[1] 朱梅刚[1] 

机构地区：[1]第一军医大学病理学教研室,广州510515

出　　处：《中华医学杂志》2002年第22期1532-1535,共4页National Medical Journal of China

基　　金：国家自然科学基金资助项目 (3 9970 692 )

摘　　要：目的　探讨经典型霍奇金淋巴瘤 (cHL)中H/RS细胞和背景淋巴细胞B细胞特异性激活蛋白 (BSAP)的表达及其意义。方法　用免疫组化方法对 33例cHL和 2 9例对照病例 (9例反应性增生的淋巴结、10例B细胞性淋巴瘤、10例T细胞性淋巴瘤 )进行BSAP检测 ,同时对cHL病例进行CD2 0、CD15、CD30常规对比检测。结果　cHL中H/RS细胞BSAP的表达率为 90 91% ,背景B淋巴细胞表达率为 10 0 % ;对照病例中反应性增生淋巴结的B淋巴细胞和B细胞性淋巴瘤肿瘤细胞的BSAP表达均为 10 0 % ,T细胞性淋巴瘤的肿瘤细胞不表达BSAP ;CD2 0表达率为 30 30 % ,BSAP和CD2 0的表达率差异有显著意义 (P =0 0 0 0 ) ;CD30表达率为 93 94 % ,CD15表达率为 75 75 % ,两者的表达率差异无显著意义 (P =0 0 82 )。结论　BSAP在H/RS细胞上表达为H/RS细胞来源于B细胞提供了进一步证据 ,并有助于H/RS细胞的辨别和鉴别。Objective To explore the expression of B cell specific activator protein (BSAP) of H/RS cell in classical Hodgkin′s lymphoma(cHL). Methods Immunohistochemical method was used to detect the expression of BSAP in 33 samples of formalin fixed, paraffin embedded tissues of cHL. Nine samples of lymph node of reactive hyperplasia, 10 samples of B cell lymphoma, and 10 samples of T cell lymphoma were also detected as BSAP controls. Mouse anti human monoclonal antibodies CD20, CD30 and CD15 were detected among the cHL cases as routine comparison. Results 30 of 33(90.91%) cases of cHL were BSAP expression positive in H/RS cells, while all of the 33(100%) cases of background B lymphocytes were BSAP positive. Almost all B cells of lymph node reactive hyperplasia were BSAP positive. All malignant cells in B cell lymphoma were BSAP positive, while all malignant cells in T cell lymphoma were BSAP negative. Among the 33 cases of cHL there was a significant difference between the expression of BSAP and the expression of CD20 (30.30%) in H/RS cells ( P = 0.000), and no significant difference between the expression of CD30 (93.94%) and CD15 (75.75%, P =0.082). Conclusion The frequent expression of BSAP in H/RS cells of classical Hodgkin′s disease provides further evidence for its B cell origin and helps to identify H/RS cells. The expression of BSAP in H/RS cells can be used to distinguish HL from anaplastic large cell lymphoma (ALCL).

关 键 词：B细胞特异性激活蛋白 经典型霍奇金淋巴瘤 淋巴细胞 免疫组织化学 CHL 基因表达 

分 类 号：R733.1[医药卫生—肿瘤]