产木聚糖酶嗜碱细菌的筛选及产酶条件研究  

Study on screening and fermentation conditions of xylanase-producing alkalophilic bacteria

作　　者：韩晓芳[1] 郑连爽[1] 等 

机构地区：[1]武汉大学资源与环境科学学院，武汉430072

出　　处：《环境污染治理技术与设备》2002年第11期25-27,共3页Techniques and Equipment for Environmental Pollution Control

基　　金：华南理工大学制浆造纸工程国家重点实验室访问学者基金项目资助

摘　　要：利用透明圈法筛选到一株产木聚糖酶的嗜碱芽孢杆菌NT 9,该菌株产木聚糖酶为组成型。正交设计实验结果表明 ,合适的产酶条件为 :木糖 15 .0g/L ,硫酸铵 2 .5 g/L ,Tween 80 2 0 g/L ,K2 HPO4 1.0g/L ,MgSO4 ·7H2 O 0 .2g/L ,pH值 10 .0 ,3 7℃ ,2 0 0r/min ,振荡培养 72h。其木聚糖酶活力可达到 6.3 6IU/mL。An alkalophilic Bacillus NT-9 was screened by assaying transparent zone on the selective medium. Through a series of orthogonal experiments, effects of carbon source, nitrogen source and other factors on xylanase production of Bacillus NT-9 were studied. The result shows that the optimal conditions for the enzyme production were xylose 1.5%, (NH 4) 2SO 4 0.25%, Tween-80 0.2%,K 2HPO 4 0.1%, MgSO 4·7H 2O 0.02%, with 72 h cultivation at pH 10.0 and 37 ℃. Under such a condition, the enzyme activity can reach 6.36 IU/mL.

关 键 词：嗜碱芽孢杆菌 木聚糖酶 正交试验 漂白技术 造纸工业 

分 类 号：TS727[轻工技术与工程—制浆造纸工程] TQ925[轻工技术与工程—发酵工程]