人宫颈癌组织染色体部分位点杂合性丢失  

Loss of heterozygosity on chromosome loci in human cervical carcinoma

作　　者：赵旻[1] 伍欣星[1] 

机构地区：[1]武汉大学医学院病毒所分子生物学教研室,武汉430071

出　　处：《基础医学与临床》2002年第6期512-516,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金 (30 1710 4 2 ) ;湖北省自然科学基金 (97J0 77)

摘　　要：了解人宫颈癌组织中 3、6、11和 18号染色体部分位点杂合性丢失的分布状况 ,为宫颈癌相关基因的定位以及临床诊断分子标志的筛选提供依据。采用宫颈癌基因组中 3、6、11和 18号染色体上的 8个微卫星标志 ,对源自宫颈癌高发区的宫颈癌活检标本 ,以PCR 变性电泳 银染的方法检测上述位点的杂合性丢失。其中在染色体 3p14、18q2 1等位点存在较高频率的杂合性丢失。结果表明杂合性丢失是宫颈癌癌变过程中常见的遗传性改变 ,宫颈癌中存在杂合性丢失的高频区 ,提示相应位点存在潜在的抑癌基因。This study was designed to investigate the loss of heterozygosity on chromosome 3,6,11 and 18 from cervical carcinoma tissues and to provide evidence for discovering new tumor suppressor genes and screening diagnostic molecular mark of cervical carcinoma. Loss of heterozygosity was formed by eight polymorphic microsatellite markers on chromosome loci 3,6,11 and 18 with PCR based silver stain method in cervical carcinoma from high incidence area of china. In 50 cervical carcinoma samples, LOH was detected of all sites with different frequencies, D3S1478 (3p21.3 21.2, 31.7%), D3S1766(3p14.3 21.1, 15%), D6S260 (6p23, 23.3%), D11S925 (11q23 24, 17.9%), D18S35 (18q21.1 21.31, 8.7%), D18S474 (18q21.1, 40.5%), D18S64 (18q21.32, 16.7%), D18S68 (18q22.1, 27.3%). LOH was detected significantly more frequently at 3p and 18q. Different frequencies of LOH on specific chromosomal regions were found. LOH was a frequent event occurring in the pathogenesis of cervical carcinoma. The results of the current study showed strongly that the putative suppressor gene(s) involved in cervical tumorigenesis was likely to locate at some specific chromosome region.

关 键 词：宫颈癌 微卫星DNA 杂合性丢失 

分 类 号：R737.33[医药卫生—肿瘤]