羧甲基壳聚糖对玉米的生理调节功能初探  

Primary Study on Physiological Regulation Function of N-Carboxmethylchitosan in Maize

作　　者：师素云 薛启汉 陈游 王学臣[2] 陈洁[3] 

机构地区：[1]江苏省农科院,江苏省南京市孝陵卫50号210014 [2]北京农业大学生物学院 [3]无锡轻工业学院食品科学与工程系

出　　处：《中国农业大学学报》1997年第5期1-6,共6页Journal of China Agricultural University

基　　金：国家攀登计划项目!"主要农作物高产高效及抗逆性生理基础研究";江苏省科委应用基础部分资助

摘　　要：以不同浓度的羧甲基壳聚糖（NCMC）处理玉米种子，其种子发芽势、发芽率、幼苗株高及幼苗叶片的叶绿素含量均有不同程度提高；用NCMC处理玉米种子及幼苗，萌发种子胚乳中α-淀粉酶、幼苗茎叶中硝酸还原酶活性分别提高45％和60％，但幼苗蛋白水解酶活性降低40％，NCMC处理开花期玉米果穗和花丝，收获的种子中贮藏蛋白含量平均提高20％左右，其中主要组分醇溶蛋白（zein）含量提高近50％。这一初步结果表明，羧甲基壳聚糖对玉米的碳、氮代谢具有一定生物调节功能，作为一种天然生物调节剂，NCMC有可能在很多作物，特别是那些蛋白质含量较低的作物中具有广泛应用前景。Corn seeds were treated with different concentration of N-car-boxymethylchitosan (NCMC) to increase seeds germinating energy, germination percentage, seedling height and chlorophyll content of seedling leaves on some extent. At flowering stage, corn ears and silk were also treated with NCMC to increase seeds storage protein content by 20% on average. Among the main fractions of corn storage protein, zein was increased nearly by 5 0 %. Enzymatical analysis indicated that α - amylase activity in germinated endosperm and nitratase activity in seedling under NCMC treatment was increased up to 45% and 60% respectively. However, protease activity in NCMC treated seedling was decreased nearly by 40% than that of control. This primary results demonstrated that N-carboxymethylchitosanpresents certain bioregulating effects on carbohydrate and nitrogen metabolism 0f maize which might have great potential to be used as a natural bioregulator widely applied in many crops particularly in protein deficiency species.

关 键 词：羧甲基壳聚糖 玉米 生理调节功能 Α-淀粉酶 硝酸还原酶 蛋白水解酶 贮藏蛋白 种子处理 

分 类 号：S482.8[农业科学—农药学] S351.1[农业科学—植物保护]