桑根氧化白藜芦醇的分离、稳态化包埋及其酪氨酸酶活性抑制作用研究  

The Separation of Oxyresveratrol from Mulberry Root and Its Effect on Steady-state Embedding and Inhibition of Tyrosinase Activity

作　　者：刘果[1] 杜洁[1] 刘飞 徐鸿 陈海 唐健 华洋林 刘光荣 曹庸[1] 

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]广州绿萃生物科技有限公司,广东广州510642 [3]广东泰禾医药科技有限公司,广东广州510000 [4]广东省茂名市林业局,广东茂名525000 [5]无限极(中国)有限公司,广东广州510665

出　　处：《现代食品科技》2015年第4期217-221 101,共6页Modern Food Science and Technology

摘　　要：氧化白藜芦醇是一种重要的活性天然产物,是反式二苯乙烯类天然产物白藜芦醇(Resveratrol)的2'-位羟基化衍生物,具有美白、抗癌、保肝、抗氧化、抗衰老、抗炎等作用。本研究在建立了精确检测氧化白藜芦醇高效液相色谱(HPLC)方法基础上,以桑根为原料,研究了氧化白藜芦醇的提取分离、稳态化包埋及对酪氨酸酶活性的抑制作用。结果表明:以80%乙醇为提取溶剂,在提取温度70℃,时间1.5 h,液料比4:1,提取次数3次条件下,氧化白藜芦醇提取得率为94.32%;在固形物含量为40%,进风温度为190℃,雾化盘转速为16000 r/min,进料速度为1.78 L/h条件下,氧化白藜芦醇微胶囊稳态化包埋率为98.91%;氧化白藜芦醇标准品、氧化白藜芦醇微胶囊样品具有对酪氨酸酶活性抑制作用,且抑制强弱呈剂量依赖型。Oxyresveratrol is a 2'-hydroxyl derivative of resveratrol(a trans-stilbene); it is an important natural bioactive product with whitening, anticancer, antioxidant, hepatoprotective, anti-inflammatory, and anti-aging functions. In this study, high-performance liquid chromatography(HPLC) was used to detect oxyresveratrol in mulberry root, and its extraction, separation, steady-state embedding, and inhibitory effects on tyrosinase activity were examined. The extraction rate of oxyresveratrol was 94.32% for the following conditions: 80% ethanol as the extraction solvent, an extraction temperature of 70 ℃, an extraction duration of 1.5 h, a liquid to solid ratio of 4:1, and independent extraction of three times. The steady-state embedding rate of oxyresveratrol was 98.91% for the following conditions: a solid content of 40%, an inlet temperature of 190 ℃, an atomizing disk speed of 16,000 r/min, and a feed speed of 1.78 L/h. The oxyresveratrol standard and oxyresveratrol microcapsule samples both had inhibitory effects on tyrosinase activity, and the strength of inhibition was dose-dependent.

关 键 词：桑根 氧化白藜芦醇 提取分离 稳态化包埋 抑制酪氨酸酶活性 

分 类 号：TS201.4[轻工技术与工程—食品科学]