E2F decoy对培养的人视网膜色素上皮细胞增生的影响  

The Influence of E2F Decoy on Proliferation of Cultured Human Retinal Pigment Epithelial Cells

作　　者：李晓青[1] 曾水清[1] 陈建斌[2] 徐莉莉[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,武汉430022 [2]华中科技大学同济医学院附属同济医院眼科,武汉430030

出　　处：《华中科技大学学报（医学版）》2002年第6期687-689,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：为探讨转录因子 E2 F decoy策略对培养的人视网膜色素上皮 (RPE)细胞增生的抑制作用 ,在阳离子脂质体L ipofectamine TM2 0 0 0介导下将 1μm ol/ L E2 F decoy或无义 decoy转入人 RPE细胞中 ,用 MTT法检测其对细胞增生活性的影响 ,RT- PCR检测细胞中 PCNA m RNA表达水平的变化。结果显示 :转录因子 E2 F decoy能抑制 RPE细胞的增生 ,为增生性玻璃体视网膜病变 (PVR)To investigate the growth inhibition of E2F on the cultured human retinal pigment epithelia (RPE), 1 μmol/L E2F decoy or scrambled decoy ODNs was introduced into human RPE by cationic liposome Lipofectamine TM 2000 and their influence on cell proliferative activity was measured by MTT assay. The change of mRNA expression of PCNA was detect by RT PCR. Results indicated that transcription factor E2F decoy could inhibit the proliferation of RPE, which provided a new gene therapy strategy for the treatment of proliferative vitreoretinopathy.

关 键 词：细胞增生 转录因子 基因治疗 E2F 视网膜色素上皮细胞 增生性玻璃体视网膜病变 

分 类 号：R774.1[医药卫生—眼科]