杨树桑黄与紫孢侧耳共培养胞内多糖提取工艺优化及抗氧化活性分析  

Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus

作　　者：陆源添 刘迪[1,2] LU Yuantian;LIU Di(Agricultural College,Yanbian University,Yanji 133002,China;Institute of Edible and Medicinal Fungi,Agricultural College,Yanbian University,Yanji 133002,China)

机构地区：[1]延边大学农学院,吉林延吉133002 [2]延边大学农学院食(药)用真菌研究所,吉林延吉133002

出　　处：《食品工业科技》2025年第2期208-217,共10页Science and Technology of Food Industry

基　　金：国家重点研发项目(2017YFD0300104);中韩国际农业技术合作项目(延大横向[2019]第19号)。

摘　　要：本研究旨在探究超声波协同半仿生法提取杨树桑黄与紫孢侧耳液体共培养胞内多糖(CC-IPS)的工艺。通过单因素实验、Plackett-Burman设计和响应面设计试验,探究第1、2、3次的提取料液比、提取时间、提取温度和提取液pH这12个因素对CC-IPS提取量的影响,以DPPH和ABTS^(+)自由基清除率评价CC-IPS的体外抗氧化能力。结果表明:最佳提取工艺参数为第1、2、3次提取料液比分别为1:17、1:40、1:30(g/mL),第1、2、3次提取时间分别为31、30、30 min,第1、2、3次提取温度分别为44、40、50℃,第1、2、3次提取液pH分别为3、8、10。在此最优工艺条件下,CC-IPS提取量为86.4921±1.1924 mg/g,与预测值85.3981 mg/g接近。CCIPS对DPPH和ABTS^(+)自由基清除率的IC_(50)分别为0.2425和0.1376 mg/mL。该研究为真菌液体共培养胞内多糖的综合高效利用开发提供理论基础。In this study,an ultrasonic synergistic semi-bionic method was explored to extract intracellular polysaccharides from Sanghuangporus vaninii and Pleurotus sapidus liquid co-cultures.By conducting single-factor experiments,Plackett-Burman design,and response surface design,the impact of 12 factors on co-culture intracellular polysaccharide(CC-IPS)extraction content,including extraction material-liquid ratios,extraction time,extraction temperature,and extraction solution pH were investigated.The antioxidant capacity of CC-IPS in vitro was evaluated by the DPPH and ABTS+radical scavenging rates.Results showed that,the optimal extraction process parameters for the first,second,and third extractions,respectively,included material-to-liquid ratios of 1:17,1:40,and 1:30(g/mL),extraction times of 31,30,and 30 min,extraction temperatures of 44,40,and 50℃,and extraction solution pH levels of 3,8,and 10.Under these optimal conditions,the CC-IPS extraction content was 86.4921±1.1924 mg/g,closed to the predicted value of 85.3981 mg/g.Additionally,the IC50 values of CC-IPS in the DPPH and ABTS+free radical scavenging assays were determined to be 0.2425 and 0.1376 mg/mL,respectively.This study offers a theoretical foundation for the efficient utilization of intracellular polysaccharides in liquid fungal co-cultures.

关 键 词：杨树桑黄 紫孢侧耳 半仿生提取法 共培养 多糖 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]