黑豆-乳清双蛋白膳食对脂多糖诱导肠屏障损伤大鼠的保护作用  

Protective Effect of Black Bean-Whey Double Protein Diet on Intestinal Barrier Injury Induced by Lipopolysaccharide in Rats

作　　者：孟维洪 李志明 张家瑜 舒欣 梁得福 庄柯瑾 张东杰[1,2,3] MENG Weihong;LI Zhiming;ZHANG Jiayu;SHU Xin;LIANG Defu;ZHUANG Kejin;ZHANG Dongjie(Heilongjiang Bayi Agricultural University,College of Food Science and Technology,Daqing 163319,China;National Coarse Grain Engineering Research and Technology Center,Daqing 163319,China;Heilongjiang Key Laboratory of Agricultural Products Processing and Quality Safety,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]国家杂粮工程技术研究中心,黑龙江大庆163319 [3]黑龙江省农产品加工与质量安全重点实验室,黑龙江大庆163319

出　　处：《食品工业科技》2025年第2期324-333,共10页Science and Technology of Food Industry

基　　金：国家重点研发计划项目(2023YFD1202705-6);黑龙江省杂粮生产与加工优势特色学科项目(2022-78);黑龙江省自然科学基金资助项目(LH2024C087);黑龙江省“优秀青年教师基础研究支持计划”资助项目(YQJH2024168)。

摘　　要：本研究旨在探讨黑豆-乳清双蛋白(Black Bean-Whey Double Protein, B-WDP)膳食对脂多糖(Lipopolysaccharide,LPS)诱导肠屏障损伤大鼠的保护作用,以SD大鼠为试验对象,饲喂低、中、高剂量的BW DP对其进行28 d膳食干预,随后采用脂多糖诱导大鼠肠屏障损伤。通过H&E染色观察大鼠的肠道形态、免疫组化检测紧密连接蛋白表达量、实时荧光定量PCR对TLR4、MyD88的表达水平进行测定。结果表明,与低剂量和高剂量B-W DP相比,中剂量的B-W DP能够极显著(P<0.01)地对LPS引起的肠屏障损伤起到预保护作用,使绒毛长度(Vuff Length,VL)数值增加26.69%,隐窝深度(Crypt Depth,CD)数值降低22.61%,二者比值提升了46.78%;三种剂量的B-W DP均能够对紧密连接蛋白起到不同的调节作用,中剂量干预效果最显著(P<0.01),使ZO-1、Claudin-1、Occludin的表达量分别提高了14.32%、31.80%、16.67%;三种剂量的B-W DP均能极显著(P<0.01)地抑制炎症传导途径,中剂量效果最明显,使TLR4和MyD88的表达水平分别降低了37.25%和33.04%。综上,中剂量B-W DP可改善肠道形态,提高肠道的消化吸收能力,增加紧密连接蛋白的表达量,维持上皮细胞完整,降低肠道通透性,下调TLR4和MyD88的表达水平,减轻炎症反应,对LPS诱导的肠屏障损伤产生了有效的保护作用。To explore the protective effects of a black bean-whey double protein(B-W double protein,B-W DP)diet on intestinal barrier injury induced by lipopolysaccharides(LPS)in rats.Sprague-Dawley rats were fed low,medium,and high doses of B-W DP for 28 d,and then,LPS was used to induce intestinal barrier injury.The intestinal morphology of the rats was observed using hematoxylin and eosin staining.The expression of tight junction proteins was quantified using immunohistochemistry,and the TLR4 and MyD88 expression levels were determined using real-time fluorescence quantitative polymerase chain reaction.Results showed that compared with low-and high-dose B-W DP,intermediate-dose B-W DP significantly(P<0.01)protected against intestinal barrier injury caused by LPS,increased villus length by 26.69%,reduced crypt depth by 22.61%,and increased their ratio by 46.78%.Three doses of B-W DP regulated tight junction proteins in different manners,and the intermediate dose exhibited the most significant effect(P<0.01),increasing ZO-1,Claudin-1,and Occludin expression levels by 14.32%,31.80%,and 16.67%,respectively.Three doses of B-W DP significantly inhibited the inflammatory pathway(P<0.01),and the intermediate dose exhibited the most significant effect,reducing TLR4 and MyD88 protein levels by 37.25%and 33.04%,respectively.In summary,a moderate dose of B-W DP can improve intestinal morphology,intestinal digestion,and absorption capacity,increase the expression of tight junction proteins,maintain the integrity of epithelial cells,reduce intestinal permeability,downregulate TLR4 and MyD88 protein expression,and alleviate inflammatory reactions.All of which exhibit effective protective effects on LPS-induced intestinal barrier injury.

关 键 词：黑豆-乳清双蛋白 脂多糖 肠屏障损伤 肠道形态 紧密连接蛋白 

分 类 号：TS201.4[轻工技术与工程—食品科学]