麦麸可溶性膳食纤维挂面对肠道菌群的影响  

Effects of wheat bran soluble dietary fiber noodles on intestinal flora

作　　者：郭瑞芳 周站杰 周雨轩 李力[1] GUO Rui-fang;ZHOU Zhan-jie;ZHOU Yu-xuan;LI-Li(College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450001,Henan,China)

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001

出　　处：《粮食与油脂》2025年第1期139-144,162,共7页Cereals & Oils

基　　金：中国博士后科学基金项目(2023M741513);河南省博士后科研资助项目(313517);河南省科技研发计划联合基金项目(232103810066、232103810066);河南省高校科技创新人才项目(23HASTIT033)。

摘　　要：为探究麦麸可溶性膳食纤维(WBSDF)对肠道菌群的调节作用,将不同添加量的WBSDF加入到小麦粉中制备WBSDF挂面,对其进行体外动态胃肠消化和体外发酵,测定发酵液的pH和短链脂肪酸(SCFAs)含量,并对其进行Miseq高通量测序分析。结果表明:面条中的WBSDF能显著降低发酵液的pH(P<0.05),产生大量的SCFAs。与未添加WBSDF组相比,WBSDF的添加能降低肠道菌群的多样性,改变肠道菌群结构。在门水平上,WBSDF的添加能显著减少厚壁菌门和放线菌门的相对丰度(P<0.05),显著增加拟杆菌门和变形菌门的相对丰度(P<0.05);在属水平上,WBSDF的添加能显著增加肠道有益菌的相对丰度(P<0.05),降低有害菌的相对丰度(P<0.05)。总体表明,WBSDF挂面能有效调节肠道菌群的结构,维持肠道健康。In order to explore the regulatory effect of wheat bran soluble dietary fiber(WBSDF)on in-testinal flora,WBSDF noodles were prepared by adding different amounts of WBSDF to wheat flour,which were subjected to in vitro dynamic gastrointestinal digestion and in vitro fermentation.The pH and short-chain fatty acids(SCFAs)content of the fermentation broth were determined,and Miseq high-throughput sequencing analysis was performed.The results showed that WBSDF in noodles could signifi-cantly reduce the pH of the fermentation broth(P<0.05)and produce a large amount of SCFAs.Com-pared with the group without WBSDF,the addition of WBSDF could reduce the diversity of intestinal flo-ra and change the structure of intestinal flora.At the phylum level,the addition of WBSDF could signifi-cantly reduce the relative abundance of Firmicutes and Actinobacteriota(P<0.05),and significantly in-crease the relative abundance of Bacteroidota and Proteobacteria(P<0.05).At the genus level,the ad-dition of WBSDF could significantly increase the relative abundance of intestinal beneficial bacteria(P<0.05)and reduce the relative abundance of harmful bacteria(P<0.05).Overall,WBSDF noodles could effectively regulate the structure of intestinal flora and maintain intestinal health.

关 键 词：麦麸可溶性膳食纤维 体外发酵 肠道菌群 高通量测序 

分 类 号：TS213.24[轻工技术与工程—粮食、油脂及植物蛋白工程]