TMT-based quantitative proteomics reveals the potential mechanism of the FufangMuji Granules in carbon tetrachloride-induced liver injury  

作　　者：Lei Men Ya-Qi Shi Xin-Yue Wang Ke-Ke Li Zhong-Yu Li Chun-Bin Li Xiao-Jie Gong 

机构地区：[1]Department of Biological Engineering,College of Life Science,Dalian Minzu University,Dalian 116600,China [2]Key Laboratory of Biotechnology and Bioresources Utilization,Ministry of Education,Dalian Minzu University,Dalian 116600,China

出　　处：《Traditional Medicine Research》2025年第4期21-30,共10页TMR传统医学研究

基　　金：National Natural Science Foundation of China(82104532 and 82173913);Joint Program(Applied Basic Research Project)of Liaoning Provincial Science and Technology Program(2023JH2/101700074);High-level Talent Innovation Support Program of Dalian(2021RD10);Fundamental Research Funds for the Central Universities(2023-SWGC-0101)。

摘　　要：Background:Fufang Muji Granules is a traditional Chinese medicine of the Manchu ethnic group and is thought to treat hepatitis and liver injury by inhibiting the elevation of alpha-fetoprotein.Methods:In this investigation,tandem mass tag(TMT)-based quantitative proteomics was performed to figure out the therapeutic mechanisms of Fufang Muji Granules on liver injury caused by carbon tetrachloride(CCl_(4))in rats.Results:Biochemical analyses(alanine aminotransferase;glutamate aminotransferase;aspartate aminotransferase)and histologic analyses(hematoxylin-eosin)demonstrated that FMG was effective in ameliorating liver injury.A sum of 6,208 proteins were identified and 2,475 proteins were determined as differential abundance proteins(DAPs)in rat liver treated with Fufang Muji Granules which compared to the model group.Bioinformatics analysis indicated that the DAPs are primarily enriched in multiple pathways such as rno00280(valine,leucine,and isoleucine degradation),rno00640(Propanoate metabolism),and rno00380(Tryptophan metabolism).Western blot was employed to validate the findings from the proteomic analysis.Conclusion:This study not only provides useful information on the mechanism of Fufang Muji Granules in the treatment of liver injury but also serves as a basis for further study of Fufang Muji Granules in vivo.

关 键 词：PROTEOMICS Fufang Muji Granules liver injury traditional Chinese medicine 

分 类 号：R57[医药卫生—消化系统]