地黄枯草芽孢杆菌全基因组测序及其促生作用相关基因研究  

The Complete Gene Sequencing of Bacilus subtilis Isolated from Rehmannia glutinosa and Study of Genes Related to Growth⁃promoting Effects

作　　者：腊贵晓[1] 王林林 郭军旗 赵玉龙 郭红霞[1] 贾慧 杨铁钢[1] LA Guixiao;WANG Linlin;GUO Junqi;ZHAO Yulong;GUO Hongxia;JIA Hui;YANG Tiegang(Institute of Chinese Herbel Medicines,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;College of Medicine,Henan University of Chinese Medicine,Zhengzhou 450046,China;Qinyang Agricultural Technology Promotion Center,Qinyang 454550,China)

机构地区：[1]河南省农业科学院中药材研究所,河南郑州450002 [2]河南中医药大学药学院,河南郑州450046 [3]沁阳市农业技术推广中心,河南沁阳454550

出　　处：《河南农业科学》2024年第12期75-85,共11页Journal of Henan Agricultural Sciences

基　　金：河南省现代农业产业技术体系项目(HARS-22-11-G2);国家现代农业产业技术体系项目(CARS-21);河南省重点研发专项(231111110800)。

摘　　要：为了探究地黄枯草芽孢杆菌(Bacilus subtilis)对地黄促生作用的相关基因,对其进行全基因组测序,并通过功能注释挖掘促生作用相关基因。结果表明,地黄内生促生菌枯草芽孢杆菌基因组大小为4310 699 bp,GC含量为43.44%,共预测编码基因4 521个。GO、COG、KEGG、CAZy、CARD和VFDB数据库注释的基因数分别为3013、3354、4395、206、52、212。与促生作用有关基因有46个,这些基因主要集中在溶磷、固氮、铁载体等方面。同时,antiSMASH预测到共409个基因富集到12个次级代谢产物合成基因簇,主要编码合成丰原素、3-羧酸碳青霉烯、类双硫醇、杆菌溶素、羊毛硫类细菌素、表面活性肽、美好菌素酸和儿茶酚型嗜铁素等8种抑菌物质,其中美好菌素酸和儿茶酚型嗜铁素还具有螯合周围环境铁离子的作用。To investigate the genes associated with the growth-promoting effects of Bacillus subtilis in Rehmannia glutinosa,whole genome sequencing was performed in this study and the genes associated with the growth-promoting effects were discovered through functional annotation.The results showed that the genome size of Bacillus subtilis,an endophytic growth-promoting bacterium of Rehmannia glutinosa,was 4310699 bp,with a GC content of 43.44%,and a total of 4521 predicted coding genes.A total of 3013,3354,4395,206,52,and 212 functional genes were annotated in the clusters of orthologous groups(COG),gene ontology(GO),kyoto encyclopedia of genes and genomes(KEGG),carbohydrate-active enzymes(CAZyme),comprehensive antibiotic resistance database(CARD),and virulence factor database(VFDB),respectively.The annotation results indicated that there were 46 genes related to growth-promoting function,with the majority of these genes involved in phosphorus solubilization,nitrogen fixation,and iron carrier processes.In the meantime,anti-SMASH predicted that a total of 409 genes were enriched into 12 secondary metabolite synthesis gene clusters,with the majority encoding eight repressors,including fengycin,1-carbapen-2-em-3-carboxylic acid,bacillaene,bacilysin,subtilosin A,surfactin,pulcherriminic acid,and bacillibactin.Among these,pulcherriminic acid and bacillibactin had been observed to facilitate the chelation of ferric ions in the surrounding environment.

关 键 词：枯草芽孢杆菌 地黄 全基因组测序 功能注释 内生菌 促生基因 

分 类 号：S567[农业科学—中草药栽培]