Protective effect of Camellia nitidissima Chi onγrays radiation-induced hematopoietic and gastrointestinal damage  

作　　者：Zhiyun Wang Haihua Shang Wenfeng Gou Feifei Xu Yue Hou Gaiting Liu Zhonghao Ren Yiliang Li Yuhua Tian Wei Li Yuefei Wang Wenbin Hou 

机构地区：[1]Institute of Radiation Medicine,Peking Union Medical College&Chinese Academy of Medical Sciences,Tianjin,China [2]National Key Laboratory of Chinese Medicine Modernization,State Key Laboratory of Component-based Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin,China [3]Shenyang Pharmaceutical University,Shenyang,China [4]Faculty of Pharmaceutical Sciences,Toho University,Funabashi,Chiba,Japan

出　　处：《Acupuncture and Herbal Medicine》2024年第4期487-499,共13页针灸和草药(英文)

基　　金：sponsored by the National Natural Science Foundation of China(82303681 and 82202950);Fundamental Research Funds for the Central Universities(3332022063);the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2021-I2M-1-042);The National Natural Science Foundation of China(82202950);the Fundamental Research Funds for the Central Universities(3332022063);Feifei Xu,who participated in the research design.The National Natural Science Foundation of China(82303681);Wenfeng Gou,who participated in the research design;The Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2021-I2M-1-042);Wenbin Hou,who designed and conceived the study.

摘　　要：Objective:Camellia nitidissima Chi,a Chinese medicine commonly used by ethnic minorities in Guangxi,China,is beneficial for clearing heat,detoxifying,inducing diuresis,and suppressing swelling.It has various pharmacological properties,including antitumor,anti-inflammatory,and antioxidant.However,its potential application in radioprotection remains unclear.In this study,we aimed to determine whether Camellia nitidissima Chi has radioprotective effects against radiation-induced gastrointestinal and hematopoietic damage.Methods:The 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)techniques were used to assess the ability of Camellia nitidissima Chi to scavenge free radicals.We conducted a 30-day survival rate experiment to evaluate the radioprotective capabilities of Camellia nitidissima Chi.Additionally,we developed models of radiation-induced intestinal and hematopoietic damage.Alterations in the white blood cell(WBC)count,total superoxide dismutase(T-SOD),glutathione(GSH),and protein expression linked to apoptosis were observed.Results:Camellia nitidissima Chi scavenged 84.72%and 93.47%of DPPH and ABTS,had a certain radiation protection potential,and increased the survival rate of mice to over 90%.Moreover,following exposure,Camellia nitidissima Chi enhanced WBC,T-SOD,and GSH levels.Camellia nitidissima Chi increased B-cell lymphoma-extra large(BCL-XL)expression and suppressed Bcl-2 associated X protein(BAX)expression,providing radioprotection to cells.Conclusions:Camellia nitidissima Chi has a strong antioxidant ability;it can improve the survival rate of mice after lethal dose irradiation and protect against radiation-induced hematopoietic and gastrointestinal damage.These findings can serve as a guide for using Chinese medicines for radioprotection.

关 键 词：Antioxidant capacity Camellia nitidissima Chi Gastrointestinal system Hematopoietic system RADIOPROTECTION 

分 类 号：R73[医药卫生—肿瘤]