The Hemerocallis citrina extracts ameliorate radiation-induced ferroptosis in LO2 cells through the Nrf2-xCT/GPX4 pathway  

作　　者：Zihao Zhu Yan Wang Zhengzheng Deng Pengyuan Lei Qi Liu Jinjing Guo Qiancheng Qing Bo Huang 

机构地区：[1]College of Public Health,Hengyang Medical School,University of South China,Hengyang,China

出　　处：《Acupuncture and Herbal Medicine》2024年第4期513-524,共12页针灸和草药(英文)

基　　金：supported by the Natural Science Foundation of Hunan Province(2021JJ30592);Health Commission Scientific Research Project of Hunan Province(D202309037942);Key Research Project of Education Department of Hunan Province(19A429);National Natural Science Foundation of China(81272994).

摘　　要：Background:Radiotherapy,a primary approach in cancer treatment,damages normal cells while targeting cancer cells.Therefore,it is crucial to identify drugs with minimal side effects,high reliability,and radioprotective effects to develop novel radiotherapy strategies.Hemerocallis citrina extracts(HCE),which are derived from plants with medicinal and culinary applications,possess antioxidative and anticancer properties.Methods:In this study,we investigated the radioprotective effects of HCE on LO2 cells exposed to radiation to determine whether these effects were mediated through the nuclear factor erythroid 2–related factor 2-cystine–glutamate antiporter/glutathione peroxidase 4 pathway.Results:Cell proliferation experiments demonstrated the radioprotective effect of HCE on LO2 cells.Western blot analysis revealed that HCE regulated B-cell lymphoma protein 2-associated X,Cleaved-caspase 3,and B-cell lymphoma protein 2,thereby inhibiting radiation-induced apoptosis,which was consistent with the flow cytometry results.Conclusions:Moreover,the detection of ferroptosis-related markers indicated that HCE alleviated radiation-induced ferroptosis in LO2 cells through the nuclear factor erythroid 2–related factor 2-cystine–glutamate antiporter/glutathione peroxidase 4 pathway.These findings provide a theoretical basis for the radioprotective effects of HCE on LO2 cells and offer new insights into the development of radioprotective drugs.

关 键 词：Ferroptosis Hemerocallis citrina extract Nrf2-xCT/GPX4 pathway RADIOPROTECTION 

分 类 号：R73[医药卫生—肿瘤]