1株牛源非解乳糖链球菌的分离鉴定及其益生特性的研究  

Study of isolation and identification of a Streptococcus alactolyticus of bovine origin and its probiotic properties

作　　者：魏欣 庞小童 聂存喜[1] 张文举[1] 尹君亮[2] 白雪 陈宁 吴妍妍[1] WEI Xin;PANG Xiao-tong;NIE Cun-xi;ZHANG Wen-ju;YIN Jun-liang;BAI Xue;CHEN Ning;WU Yan-yan

机构地区：[1]石河子大学动物科技学院,新疆石河子832003 [2]新疆农垦科学院,新疆石河子832000

出　　处：《饲料研究》2024年第22期89-94,共6页Feed Research

基　　金：石河子大学高层次人才科研启动项目(项目编号:RCZK202359);兵团重大科技“揭榜挂帅”项目(项目编号:2023AB078);兵团重大科技项目(项目编号:2021AA004);兵团自然科学支持计划(项目编号:2024DB027)。

摘　　要：试验旨在从新生弗荷F1 (♂弗莱维赫牛×♀荷斯坦)的不同肠段黏膜中筛选肠道定植菌株并研究其益生特性。试验采用16S rRNA基因测序分析方法对分离菌株进行种属鉴定,并对分离鉴定的乳酸菌株进行体外益生功能的研究,综合评价分离菌株的特性。结果显示,从弗荷F1的不同肠段黏膜中分离出1株乳酸菌候选菌株,经16S rRNA测序鉴定,为非解乳糖链球菌,命名为F2。该菌株对大肠杆菌、金黄色葡萄球菌和沙门氏菌具有潜在的抑菌能力,该菌株具有较高的疏水性、自聚集性、耐酸性、胆汁耐受性和抗生素敏感性。研究表明,从牛源肠道黏膜中分离出的乳酸菌可能具有成为益生菌的潜力。The experiment aimed to screen for gut colonizing bacterial strains from different intestinal mucosal segments of the Ferhe F1cow(♂Fleckvieh×♀Holstein)and to study their probiotic properties.The experiment used 16S rRNA gene sequencing analysis to identify the species of isolated strains and conducted invitroprobiotic function research on the isolated and identified lactic acid bacterial strains to comprehensively evaluate the characteristics of the isolated strains.The results showed that one lactic acid bacterial candidate strain was isolated from the different intestinal mucosal segments of the Ferhe F1cow identified by 16S rRNA sequencing,it was a Streptococcusalactolyticus,named F2.This strain had potential inhibitory effects on Escherichia coli,Staphylococcus aureus,and Salmonella.The strain has high hydrophobicity,auto-aggregation,acid tolerance,bile tolerance,and antibiotic sensitivity.The study indicates that lactic acid bacteria isolated from bovine origin of intestinal mucosa may have the potential to become probiotics.

关 键 词：牛源 乳酸菌 分离鉴定 益生特性 

分 类 号：S816.7[农业科学—饲料科学]