肉桂精油与山苍子精油复配协同对鸡白痢沙门氏菌生物膜的影响  

Effect of compounding Cinnamomum cassia essential oil and Litsea cubeba essential oil on biofilm of Salmonella pullorum

作　　者：邢帅 谭雨箫 黄伊芹 钱雅静 张歌音 司红彬[1,2] XING Shuai;TAN Yu-xiao;HUANG Yi-qin;QIAN Ya-jing;ZHANG Ge-yin;SI Hong-bin

机构地区：[1]广西大学动物科技学院,广西南宁530004 [2]亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004

出　　处：《饲料研究》2024年第22期99-104,共6页Feed Research

基　　金：国家基金联合基金(项目编号:U22A20523);中央引导地方专项(项目编号:桂科计字[2023] 55号)。

摘　　要：试验旨在研究肉桂精油(CEO)与山苍子精油(LEO)对沙门氏菌(鸡白痢沙门氏菌SP1株)的协同抑菌作用及其对生物膜的影响。通过测定最小抑菌浓度(MIC)和分级抑菌指数(FICI),检测用药前后核酸和蛋白泄漏情况及活性氧(ROS)的变化,探究CEO与LEO对SP1的抑菌作用机理。试验通过对生物膜的抑制能力、清除能力、胞外聚合物(EPS)含量的测定,探究CEO与LEO对SP1生物膜的影响。结果显示,CEO对SP1的MIC为0.39 g/L,LEO对SP1的MIC为1.56 g/L,联用FICI为0.5。复方精油可破坏SP1的细胞完整性,胞外核酸、蛋白和ROS含量均显著升高(P<0.05)。复方精油抑制SP1的生物膜形成(P<0.05)且联用24 h后显著清除生物膜(P<0.05),复方组和2×复方组的胞外多糖含量和聚集能力均显著降低(P<0.05)。研究表明,CEO与LEO对SP1株均有抑制作用,且复方精油能够破坏沙门氏菌的结构完整性,造成氧化损伤,抑制生物膜的形成,降低胞外多糖含量和细菌的聚集能力,从而实现对沙门氏菌的抑制作用。The study aimed to investigate the synergistic antibacterial effects of Cinnamomumcassiaessential oil(CEO)and Litseacubeba essential oil(LEO)against Salmonella(S.pullorum SP1)and their effects on biofilm.The minimum inhibitory concentration(MIC)and fractional inhibitory concentration index(FICI)were determined to evaluate the antibacterial activity of CEO and LEO against SP1.Changes in nucleic acid and protein leakage and reactive oxygen species(ROS)production were measured to explore the antibacterial mechanism.The effects of CEO and LEO on SP1 biofilm were evaluated by assessing biofilm inhibition,removal ability,and extracellular polymeric substance(EPS)content.The results showed that the MIC of CEO against SP1 was 0.39 g/L,while the MIC of LEO was 1.56 g/L.The FICI of the combination was 0.5.The combination of essential oils disrupted the cell integrity of SP1,leading to significant increases in extracellular nucleic acid,protein,and ROS levels(P<0.05).The combination of essential oils inhibited biofilm formation(P<0.05)and significantly reduced biofilm biomass after 24 hours(P<0.05).The extracellular polysaccharide content and aggregation ability of SP1 were significantly decreased in the combination group and 2×combination group(P<0.05).The study indicates that CEO and LEO have inhibitory effects against SP1,and the combination of essential oils can disrupt the structural integrity of Salmonella,induce oxidative damage,inhibit biofilm formation,reduce extracellular polysaccharide content,and decrease bacterial aggregation ability,thereby achieving inhibitory effects against Salmonella.

关 键 词：山苍子精油 肉桂精油 沙门氏菌 协同抑菌 生物膜 

分 类 号：S816.7[农业科学—饲料科学]