延胡索酸对紫色红曲菌生长和次级代谢的影响  

Effects of Fumaric Acid on Growth and Secondary Metabolism of Monascus purpureus

作　　者：秦昱晖 王海蛟 程颖 王聪聪[1] 孙晴 董慧君 张薄博 刘成建 王成涛[1,2] 张婵 QIN Yuhui;WANG Haijiao;CHENG Ying;WANG Congcong;SUN Qing;DONG Huijun;ZHANG Bobo;LIU Chengjian;WANG Chengtao;ZHANG Chan(School of Food and Health,Beijing Technology and Business University,Beijing 100048,China;Beijing Key Laboratory of Flavor Chemistry,Beijing 100048,China;School of Science,Shantou University,Shantou 515063,China;Shandong Fanhui Pharmaceutical Co.Ltd.,Jinan 271100,China)

机构地区：[1]北京工商大学食品与健康学院,北京100048 [2]北京市食品添加剂工程技术研究中心,北京100048 [3]汕头大学理学院,广东汕头515063 [4]山东凡卉药业有限公司,山东济南271100

出　　处：《食品与生物技术学报》2024年第10期54-64,共11页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(32172185);北京市科技新星计划项目(20240484599);北京工商大学食品科学与工程“双一流”学科建设培育项目(BTBUYXTD202208)。

摘　　要：红曲菌(Monascus)发酵可产生多种有益次级代谢产物,如Monacolin K、红曲色素等。Monacolin K可以抑制胆固醇的形成并降低血脂,但野生型红曲的Monacolin K产量极低,严重限制了功能红曲的应用。作者所在研究团队前期通过代谢组学分析发现三羧酸循环(tricarboxylic acid cycle,TAC)途径参与调控Monacolin K的合成,且延胡索酸的表达与Monacolin K合成呈正相关。为进一步探究延胡索酸对紫色红曲菌(Monascus purpureus)生长和次级代谢的影响,提高紫色红曲菌的Monacolin K产量,作者以野生型紫色红曲菌M1作为发酵菌株,通过干质量、分光光度、扫描电子显微镜、超高效液相色谱、实时荧光定量PCR等方法探究延胡索酸对红曲菌菌丝体生长及次级代谢产物Monacolin K和黄、橙、红共3种红曲色素合成等的影响。结果表明,延胡索酸的添加抑制了菌丝体的生长以及红曲色素的合成,但菌丝体褶皱程度和密度有所提高,Monacolin K产量较对照组提高了49.38%,与Monacolin K合成相关的9段基因呈现不同程度的上调趋势。Monascus fermentation can produce various beneficial secondary metabolites,such as Monacolin K,Monascus pigments,and so on.Monacolin K can inhibit cholesterol synthesis and reduce blood lipid levels.However,wild-type Monascus strains have extremely low yields of Monacolin K,significantly limiting the application of functional Monascus products.The previous metabolomics analysis of the author’s research team revealed that the tricarboxylic acid cycle(TAC)is involved in regulating Monacolin K synthesis,and fumaric acid expression was positively correlated with the synthesis of Monacolin K.To further investigate the effect of fumaric acid on the growth and secondary metabolism of Monascus purpureus and to improve Monacolin K yield in Monascus purpureus,the author utilized the wild-type strain Monascus purpureus M1 as the fermentation strain.Using methods such as dry weight measurement,spectrophotometry,scanning electron microscopy,ultrahigh-performance liquid chromatography,and real-time quantitative PCR,this author investigated the impact of fumaric acid addition on Monascus growth and the synthesis of secondary metabolite Monacolin K,as well as the yellow,orange,and red pigments of Monascus.The results demonstrated that fumaric acid addition inhibited the growth of mycelia and the synthesis of Monascus pigments.However,it increased the degree of mycelial folding and density,with Monacolin K production showing a 49.38%increase compared to the control.Additionally,nine genes associated with Monacolin K synthesis exhibited varying degrees of upregulation.

关 键 词：红曲菌 延胡索酸 Monacolin K 实时荧光定量PCR 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]