植物乳杆菌CCFM8661与水苏糖复合应用对小鼠铅中毒的缓解作用  

Alleviating Effect of Lactobacillus plantarum CCFM8661 Combined with Stachyose on Lead Toxicity in Mice

作　　者：孔庆敏 刘振国 李琴丽 张书榕 付大伟[1] 徐伟[1] 马永强[1] KONG Qingmin;LIU Zhenguo;LI Qinli;ZHANG Shurong;FU Dawei;XU Wei;MA Yongqiang(School of Food Engineering,Harbin University of Commerce,Harbin 150028,China)

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150028

出　　处：《食品与生物技术学报》2024年第10期97-108,共12页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金青年科学基金项目(32302051);哈尔滨商业大学“青年科研创新人才”培育计划项目(2023-KYYWF-1056);哈尔滨商业大学博士科研启动计划项目(22BQ83)。

摘　　要：合生元是由益生菌和益生元组成的微生物制剂,同时具有益生菌和益生元的功能。作者前期研究已经证实植物乳杆菌CCFM8661是一株具有良好铅毒性保护作用的菌株。水苏糖是一种优质益生元,具有调节肠道菌群、抗氧化以及排毒等生理功能。作者研究了植物乳杆菌CCFM8661和水苏糖组合的合生元对铅诱导的小鼠肝肾损伤的影响,探究其对小鼠组织生理生化指标、肠道菌群结构及粪便代谢产物的影响。结果表明,植物乳杆菌CCFM8661与水苏糖的复合应用显著提高了铅中毒的缓解效果,特别是高剂量组合在降低组织铅质量分数及缓解抗氧化损伤方面效果更为显著。此外,该复配干预显著改变了特定肠道细菌的丰度,如Ruminococcaceae UCG-014、Ruminococcus 1、Alistipes、Akkermansia等;并调整了肠道菌群代谢,如影响了马尿酸、3-苯丙酸、雌马酚以及多种短链脂肪酸和氨基酸等物质含量,能对铅中毒小鼠产生潜在的积极效应。该研究表明植物乳杆菌CCFM8661与水苏糖复配可能成为缓解肠道铅中毒的新制剂。Synbiotics is a microbial formulation composed of probiotics and prebiotics that can simultaneously exhibit the functions of both.The previous study of the author has confirmed that Lactobacillus plantarum(L.plantarum)CCFM8661 is a strain with significant protective effects against lead toxicity.Stachyose is a high-quality prebiotic oligosaccharide,known for its physiological functions such as regulating gut microbiota,providing antioxidant effects,and aiding in detoxification.The author investigated the effects of a synbiotic combination of L.plantarum CCFM8661 and stachyose on alleviating liver and kidney damage induced by lead exposure in mice,and explored its impacts on physiological and biochemical indicators in mouse tissues,gut microbiota structure,and fecal metabolites.The results showed that the combined application of of L.plantarum CCFM8661 and stachyose significantly enhanced the alleviation of lead poisoning,with the high-dose combination showing a more pronounced effect in reducing the lead mass fraction in tissues and mitigating antioxidative damage.Moreover,the combined intervention of L.plantarum CCFM8661 and stachyose significantly affected the abundance of specific gut bacteria such as Ruminococcaceae UCG-014,Ruminococcus 1,Alistipes,and Akkermansia.It also modulated gut microbiota metabolism,influencing the levels of hippuric acid,3-phenylpropionic acid,estrophin,as well as various short-chain fatty acids and amino acids,which may provide potential benefits for mice with lead poisoning. These findings suggested CCFM8661 compoundstachyose may serve as a novel formulation for the mitigation of intestinal lead toxicity.

关 键 词：植物乳杆菌 水苏糖 铅 肠道菌群 代谢产物 

分 类 号：TS201.3[轻工技术与工程—食品科学]