Autophagy mediated tubulobulbar complex components degradation is required for spermiation  

作　　者：Liying Wang Ruidan Zhang Bingbing Wu Yang Yu Wei Li Shiguo Li Chao Liu 

机构地区：[1]Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangzhou 510623,China [2]State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Stem Cell and Regenerative Medicine Innovation Institute,Chinese Academy of Sciences,Beijing 100101,China [3]Department of Radiology,State Key Laboratory of Cardiovascular Disease,Fuwai Hospital,National Center for Cardiovascular Diseases,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100037,China [4]College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Fundamental Research》2024年第6期1557-1567,共11页自然科学基础研究(英文版)

基　　金：supported by the National Science Fund for Distinguished Young Scholars(81925015);the Youth Innovation Promotion Association of CAS(2018109).

摘　　要：Spermiation is the process that releases mature spermatids from Sertoli cells into the lumen of the seminiferous tubule.Tubulobulbar complexes(TBCs)are elaborate cytoskeleton-related structures that are indispensable for spermiation.Despite well-defined ultrastructural events,the molecular regulation of TBCs during spermiation re-mains largely unknown.Here,we show that autophagy is active in TBC regions,and impaired autophagy in Sertoli cells affects spermiation.Further studies demonstrated that many TBC components bound to LC3 and could be selectively degraded through the autophagy-lysosome pathway.Perturbed autophagy impaired the degradation of some TBC components in Sertoli cells,such as VCL and CTTN,and led to the accumulation of TBC compo-nents surrounding the spermatid head,which may be associated with the sperm-releasing defect.Together,our results reveal that autophagy is essential for the TBC components degradation in mouse Sertoli cells and define a functional role of autophagy during spermiation.

关 键 词：AUTOPHAGY SPERMIATION Tubulobulbar complexes Cytoskeleton degradation Sertoli cells LYSOSOME 

分 类 号：TG1[金属学及工艺—金属学]