石崖茶黄酮类成分提取工艺优化及其抗病毒和抑菌活性研究  

Optimization of Extraction Process of Flavonoids from Adinandra nitida Merr.ex.H.L.Li and Antiviral and Antibacterial Activities

作　　者：陈路[1] 刘钰[1] 蒋莉 黎宗浩 张宇深 赵鹏 刘颖颖 吴无畏[1] CHEN Lu;LIU Yu;JIANG Li;LI Zonghao;ZHANG Yushen;ZHAO Peng;LIU Yingying;WU Wuwei(Guangxi Botanical Garden of Madicinal Plant,National Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development,Nanning 530023,China;Guangxi Botanical Garden of Madicinal Plant,Key Laboratory of Guangxi for High-Quality Formation and Utilization of Dao-Di Herbs,Nanning 530023,China)

机构地区：[1]广西壮族自治区药用植物园,西南濒危药材资源开发国家工程研究中心,广西南宁530023 [2]广西壮族自治区药用植物园,广西道地药材高品质形成与应用重点实验室,广西南宁530023

出　　处：《食品科技》2024年第11期220-227,共8页Food Science and Technology

基　　金：广西食品安全地方标准制修订项目(桂地标食2022008);广西科技计划项目(AD17292003);南宁市科学研究与技术开发计划项目(20212131)。

摘　　要：为优化石崖茶黄酮类成分的提取工艺,并探究其抗病毒和抗菌性能,研究采用纤维素酶辅助溶剂回流提取法对石崖茶中的黄酮类成分进行提取,以总黄酮得率及代表性成分山茶苷A和芹菜素的收率为指标,采用正交试验设计优化提取工艺,同时探究石崖茶黄酮类成分对柯萨奇病毒以及对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌等的抑制作用。结果表明,最佳提取工艺为料液比1:60、pH4.0、纤维素酶溶液0.2%、50℃酶解1 h、回流提取1.5 h,在此条件下石崖茶中黄酮类成分的得率为37.18%。石崖茶黄酮类成分中高剂量组具有较强的抗病毒及体外抑菌作用。研究结果为石崖茶的开发与利用提供了理论支持。In order to optimize the extraction process of flavonoid components from Adinandra nitida and to explore its antiviral and antibacterial properties,this study employed cellulase-assisted solvent reflux extraction to extract flavonoids from A.nitida.The total flavonoid yield and the yields of representative compounds such as adinatrin A and apigenin were used as indicators.Orthogonal test design was utilized to optimize the extraction process.Additionally,the study investigated the inhibitory effects of flavonoid components in A.nitida on Coxsackie virus B5(CVB5)and bacteria including Escherichia coli,Staphylococcus aureus,and Pseudomonas aeruginosa.The findings indicated that the optimal extraction conditions are a solid-liquid ratio of 1:60,pH value of 4.0,cellulase solution at 0.2%,enzymatic hydrolysis at 50℃for 1 hour,and reflux extraction for 1.5 hours.Under these conditions,the yield of flavonoid components in A.nitida reached 37.18%.The high-dose group of flavonoid components demonstrated potent antiviral and in vitro antibacterial activities.This research offers theoretical support for the development and application of A.nitida.

关 键 词：石崖茶 黄酮类成分 抗病毒 抑菌 正交试验设计 

分 类 号：TS201.2[轻工技术与工程—食品科学]