食药用真菌固态发酵香菇柄基质产多糖的工艺优化及抗氧化活性分析  

Optimization of Polysaccharide Production from Edible and Medicinal Fungi via Solid-State Fermentation of Lentinula edodes Stem Substrate and Its Analysis of Antioxidant Activity

作　　者：曹恰 黄文[1,2] 王益 刘莹[1,2] CAO Qia;HUANG Wen;WANG Yi;LIU Ying(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Hubei Key Laboratory of Fruit and Vegetable Processing and Quality Control,Wuhan 430070,China)

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070 [2]果蔬加工与品质调控湖北省重点实验室,湖北武汉430070

出　　处：《食品科技》2024年第11期227-233,共7页Food Science and Technology

基　　金：湖北省重点研发计划项目(2022BBA0024);湖北省农业科技创新中心农产品加工与综合利用项目(2021-620-000-001-031);湖北省食用菌产业技术体系项目(HBHZDZB-2021-023)。

摘　　要：以香菇柄为基质,研究不同食药用菌菌丝体固态发酵产多糖的工艺及其抗氧化活性。通过羊肚菌、茯苓菌、大球盖菇、草菇、香菇、灵芝、花脸菌、白参菌、猴头菌、血耳菌等菌种对香菇柄进行发酵制备多糖,筛选出最优菌种,进而采用单因素和正交试验优化制备工艺,并通过1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl,DPPH)自由基清除率评估发酵前后制得多糖的抗氧化活性。结果显示,白参菌是最优发酵菌种,其固态发酵香菇柄基质(10 g)产多糖的最优条件为发酵时间9 d、接种量12.5 mL、水分添加量1:1.5(g/mL),在此条件下,多糖的得率可高达到(16.80±0.60)%,纯度为(75.40±1.81)%。发酵后制备的香菇柄多糖对DPPH自由基具有更强的清除作用,其IC_(50)值为2.445 mg/mL。白参菌固态发酵香菇柄制备多糖能够提高其得率、纯度,并增强其抗氧化活性,该研究结果可为香菇柄多糖的制备提供理论依据,有助于香菇柄资源的开发。The study investigated the optimal process for producing polysaccharides from edible and medicinal fungi via solid-state fermentation of Lentinula edodes stem substrate and its antioxidant activity.Polysaccharides were prepared by fermentation of Lentinula edodes stem by Morchella,Poria cocos,Stropharia rugosoannulata,Volvariella volvacea,Lentinula edodes,Ganoderma lucidum,Lepista sordida,Schizophyllum commune,Hericium erinaceus,Tremella sanguinea Peng and other fungi.The optimal fungal was selected,and both single-factor and orthogonal experiments were conducted to optimize the preparation process.The antioxidant activity of the polysaccharides obtained before and after fermentation was evaluated by the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals scavenging assay.The results revealed that Schizophyllum commune was the optimal fermentation strain.The optimal conditions for solid-state fermentation of Lentinula edodes stem substrate(10 g)to produce polysaccharides were determined to be a fermentation period of 9 days,inoculum volume of 12.5 mL,and amount of water added of 1:1.5(g/mL).Under these conditions,the polysaccharide yield reached(16.80±0.60)%,with a purity of(75.40±1.81)%.The polysaccharides obtained by fermenting had a stronger scavenging effect on DPPH radical,and its IC50 value was 2.445 mg/mL.Solid-state fermentation of Lentinula edodes stem by Schizophyllum commune improved polysaccharide yield,purity,and antioxidant activity.These findings provide a theoretical basis for the preparation of polysaccharides from Lentinula edodes stem and contribute to the development of its resources.

关 键 词：香菇柄 多糖 白参菌 发酵 抗氧化 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]