油茶多肽稳定性及降血糖作用的研究  

Stability and Hypoglycemic Effect of Camellia Seed Cake Peptides

作　　者：俞遴 吴继来 李珍 俞力军 张元萍 吴峰华[3] YU Lin;WU Jilai;LI Zhen;YU Lijun;ZHANG Yuanping;WU Fenghua(Lin’an District Agriculture and Rural Bureau of Hangzhou City,Hangzhou 311300,China;Lin’an District Qingshan Lake National Park Management Bureau of Hangzhou City,Hangzhou 311300,China;College of Food and Health,Zhejiang A&F University,Hangzhou 311300,China)

机构地区：[1]杭州市临安区农业农村局,浙江杭州311300 [2]杭州市临安区青山湖国家森林公园管理局,浙江杭州311300 [3]浙江农林大学食品与健康学院,浙江杭州311300

出　　处：《食品科技》2024年第11期241-248,共8页Food Science and Technology

基　　金：浙江省“领雁”研发攻关计划项目(2023C02032);浙江省重点研发计划项目(2021C02014)。

摘　　要：以从油茶籽饼粕多肽(Camellia seed cake peptides,CSCPs)中鉴定出具有体外降血糖活性的2条肽段LLVLYYEY(CSCPs-1)和LLLLPSYSEF(CSCPs-2)为受试物,评估了它们模拟胃肠道消化、不同温度和pH值的稳定性。通过斑马鱼高血糖模型评价它们的体内降血糖效果并初步探究降血糖作用机制。结果表明,CSCPs-1和CSCPs-2经过胃消化阶段活性无显著变化,在肠消化阶段活性显著降低,具有良好的酸碱稳定性和热稳定性。与高糖模型组相比,CSCPs-1和CSCPs-2的低、中、高剂量组均能够显著降低斑马鱼体内的血糖水平,改善高糖引起糖代谢关键因子Insulin、Pck1、Pdx-1的mRNA异常表达。研究表明油茶降血糖肽CSCPs-1和CSCPs-2具有开发成为抗糖尿病功能食品的潜力。The gastrointestinal digestion,temperature,and pH stablility of two peptides,LLVLYYEY(CSCPs-1)and LLLLPSYSEF(CSCPs-2),identified from Camellia seed cake peptides(CSCPs)with hypoglycemic activity were evaluated in vitro.The hypoglycemic effect of zebrafish was evaluated by the hyperglycemic model and the mechanism of hypoglycemic action was preliminarily explored.The results showed that the activities of CSCPs-1 and CSCPs-2 did not change significantly during gastric digestion,but significantly decreased during intestinal digestion,showing good acid-base stability and thermal stability.Compared with the high glucose model group,the low,medium,and high dose groups of CSCPs-1 and CSCPs-2 could significantly reduce the blood glucose level in zebrafish and improve the mRNA abnormal expression of Insulin,Pck1,and Pdx-1,the key factors of glucose metabolism caused by high glucose.This study indicated that Camellia hypoglycemic peptides CSCPs-1 and CSCPs-2 have the potential to be developed into anti-diabetic functional foods.

关 键 词：油茶 降血糖肽 斑马鱼模型 降血糖活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]