4种鹰爪花属植物叶片提取物抗氧化活性研究  

Study on antioxidant activity of extracts from leaves of four Artabotrys species

作　　者：石婧 梁泽锐 王慧颖 张晖 薛彬娥 SHI Jing;LIANG Zerui;WANG Huiying;ZHANG Hui;XUE Bine(College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)

机构地区：[1]仲恺农业工程学院园艺园林学院,广东广州510225

出　　处：《仲恺农业工程学院学报》2024年第4期8-14,29,共8页Journal of Zhongkai University of Agriculture and Engineering

基　　金：广东省林业科技创新项目(2020KJCX010);国家自然科学基金项目(31872646).

摘　　要：为进一步开发利用番荔枝科鹰爪花属(Artabotrys)植物,为药物或食品天然抗氧化剂筛选和应用提供参考,本研究以4种鹰爪花属植物——狭瓣鹰爪花(Artabotrys hainanensis R.E.Fries)、鹰爪花(Artabotrys hexapetalus(L.f.)Bhandari)、毛叶鹰爪花(Artabotrys pilosus Merr.&Chun)、厚瓣鹰爪花(Artabotrys pachype talus B.Xue&Junhao Chen)为研究对象,通过测定总黄酮、总多酚含量,并以DPPH·清除能力、·OH清除能力和ABTS^(+)·清除能力3个指标对4种植物的叶片提取物抗氧化活性进行评价.结果表明:(1)不同植物的总黄酮含量和总酚含量差异显著,4种植物的总黄酮含量和总多酚含量分别为1.33~7.99 mg/g和2.26~9.50 mg/g,其中,毛叶鹰爪花的总黄酮和总酚含量最高;(2)4种植物均具有一定的抗氧化能力,抗氧化活性随提取物浓度增大而增强,其中毛叶鹰爪花对DPPH·和ABTS^(+)·2种自由基清除能力最强,也有较好的清除·OH自由基的能力;(3)4种植物中总黄酮含量与DPPH·和ABTS^(+)·清除能力均呈显著正相关,总酚含量与DPPH·和ABTS^(+)·清除能力均呈极显著正相关.因此综合看来,4种鹰爪花属植物中,毛叶鹰爪花叶片乙醇提取物抗氧化活性最好,最具有开发为天然抗氧化剂的潜力.In order to further develop and utilize Artabotrys plants in Annonaceae and provide reference for the screening and application of natural antioxidants in medicines or foods,four Artabotrys species(Artabotrys hainanensis,Artabotrys hexapetalus,Artabotrys pilosus,Artabotrys pachypetalus)were selected as research objects in this study.The contents of total polyphenols and total flavonoids of the ethanol extracts of the leaves were determined.The antioxidative activities of four Artabotrys species were evaluated by using the scavenging ability of DPPH·,·OH and ABTS^(+)·as three indicators.The results are as follows:(1)The contents of the total polyphenols and the total flavonoids in the leaf extracts of the four species are significantly different.The contents of total flavonoids of the four species range from 1.33 to 7.99 mg/g,and the contents of the total polyphenols of the four species range from 2.26 to 9.50 mg/g.Among the four species,A.pilosus has the highest contents of total polyphenols and total flavonoids.(2)The four extraxts all have antioxidant activity,and the antioxidant activity increases with the increase of the concentration of the leaf extracts.Among the four species,the leaf extracts of A.pilosus has the strongest radical scavenging capacity on DPPH·,ABTS^(+)·and also has good ability to scavenge·OH radicals.(3)The total flavonoids contents and the scavenging ability of DPPH·and ABTS^(+)·are positive correlated,and the total polyphenol contents and the scavenging ability of DPPH·and ABTS^(+)·are significantly positive correlated.In general,the leaf extracts of A.pilosus has the best antioxidant activity among the four Artabotrys species and has the potential to be developed as a natural antioxidant.

关 键 词：番荔枝科(Annonaceae) 鹰爪花属(Artabotrys) 抗氧化活性 总黄酮 总酚 

分 类 号：S603.7[农业科学—园艺学]