Comprehensive analyses of inhibition of the Chinese traditional food-packaging materials,Indocalamus latifolius leaves(Zongye)on Aspergillus flavus  

作　　者：Wenjing Peng Xiaoxu Ren Haiting Dai Bowen Tai Bolei Yang Gang Wang Xu Li Fuguo Xing 

机构地区：[1]College of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin,China [2]Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process,Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing,China

出　　处：《Food Quality and Safety》2024年第4期720-739,共20页食品品质与安全研究(英文版)

基　　金：supported by the Beijing Natural Science Foundation(Nos.6212028 and 6222053);the National Natural Science Foundation of China(No.32372457);the Agricultural Science and Technology Innovation Program of Institute of Food Science and Technology,CAAS(No.CAAS-ASTIP-G2022-IFST-01).

摘　　要：Objectives:The contamination of Aspergillus flavus and aflatoxins(AFs)is one of the most serious safety problems in food and feed.The discovery and application of plant-sourced antifungal agents are hot topics in mycotoxin control.In this study,we aim to develop efficient strategies to control A.flavus and subsequent contamination by AFs.Materials and Methods:We focused on Zongye essential oil,which was extracted from Indocalamus latifolius leaves(Zongye,the traditional food-packaging materials).Its antifungal and antitoxin activities were observed by volatile test,and the significant morphological and ultrastructural variations were observed by scanning electron microscopy(SEM)and transmission electron microscopy(TEM)analyses.The main components of Zongye essential oil were analyzed by gas chromatography-mass spectrometry(GC-MS).RNA sequencing(RNA-Seq)and real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR)analyses were used to reveal the inhibitory mechanism.Results:Among them,No.2 Zongye essential oil completely inhibited the growth and toxin production of A.flavus after 10μL treatment.Under Zongye essential oil treatment,evidently morphological and ultrastructural variations were observed,such as hyphae shrinkage,partial distortion,and a decrease in conidia number.Longifolene and linalool were the major constituents of Zongye essential oil,accounting for 9.55% and 7.95%,respectively,and linalool had stronger inhibitory effects on fungal growth and mycotoxin biosynthesis than longifolene.Based on the experimental results,the antifungal mechanism was proposed:down-regulations of membrane proteins(AFLA_005560,AFLA_019420,and AFLA_084310,etc.)and conidial genes(fblC,steA,and abaA,etc.)inhibited fungal development,and anti-aflatoxigenic activity might be due to significant down-regulation of AF clustergenes andglobal regulators(AtfA and AtfB).Conclusion:A novel antifungal agent,Zongye essential oil,was identified,and its inhibitory mechanism was comprehensively clarified,which is helpfu

关 键 词：Indocalamus latifolius Zongye essential oil Aspergillus flavus AFLATOXIN inhibitory mechanism 

分 类 号：TS206.4[轻工技术与工程—食品科学]