体外模拟消化环境中乳清浓缩蛋白对5-甲基四氢叶酸钙生物可及性及微观结构的影响  

Effects of Whey Protein Concentrate on the Bioaccessibility and Microstructure of 5-Methyltetrahydrocalcium Folate in Simulated Digestive Environment in Vitro

作　　者：张雨松 崔蔚然 唐祯玥 白红雨 邵美丽[1] ZHANG Yusong;CUI Weiran;TANG Zhenyue;BAI Hongyu;SHAO Meili(College of Food Science,Northeast Agricultural University,Harbin 150000,China)

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150000

出　　处：《食品工业科技》2025年第3期92-100,共9页Science and Technology of Food Industry

基　　金：中国食品科学技术学会食品科技基金——雅培食品营养与安全专项科研基金。

摘　　要：生物可及性是5-甲基四氢叶酸钙(5-methyltetrahydrocalcium folate,5-MTHF)发挥生物功能的前提,易受到食品组分的影响。该文以乳清浓缩蛋白(whey protein concentrate,WPC)和5-MTHF为研究对象,利用体外消化系统,探究WPC在不同条件下(浓度、加工方式、胃肠pH、消化时间)对5-MTHF生物可及性的影响,并采用胶体粒径电位仪与激光共聚焦观察消化前后粒子微观结构的变化。结果表明,在胃消化阶段,WPC可将5-MTHF紧密包裹,较好地保护了5-MTHF,并成功转运至肠消化阶段完成释放,提高了5-MTHF的生物可及性。同时,WPC浓度、WPC加工方式、胃肠pH、消化时间等均不同程度影响5-MTHF的生物可及性和粒径、电位。其中,在胃消化阶段,各WPC-5-MTHF组均未检测出5-MTHF,生物可及性为0。在肠消化阶段,WPC-5-MTHF组的生物可及性随WPC浓度的增加而提高,较5-MTHF组的生物可及性提高了11.1%~19.61%;超声处理组、高压均质组、加热处理组的生物可及性较未加工处理组分别减少了8.49%、9.52%、8.75%;在肠pH为7、消化时间为5 h时,WPC-5-MTHF组拥有最佳生物可及性,分别为45.17%、42.32%,与5-MTHF组相比,提高到2倍左右。粒径和电位的结果显示,WPC-5-MTHF组较WPC拥有更小的粒径,更大的电位绝对值,并且WPC-5-MTHF结构的变化会引起消化特性的改变,该研究为5-甲基四氢叶酸钙在乳制品中的应用提供理论指导。Bioaccessibility is a prerequisite for 5-methyltetrahydrocalcium folate(5-MTHF)to perform their health functions and is susceptible to the influence of food components.Through in vitro simulated digestion,the effects of whey protein concentrate(WPC)on the bioaccessibility of 5-MTHF under various circumstances(concentration,processing technique,gastrointestinal pH,and digesting duration)were examined.The changes of particle microstructure before and after digestion were observed by colloid particle size potentiometer and laser confocal.According to the findings,5-MTHF could be securely wrapped,shielded by WPC throughout the stomach stage of digestion,and then moved to the small intestine for full release.Additionally,during the digestive process,the bioaccessibility of WPC-5-MTHF tended to rise in all groups.In the meanwhile,several factors such as WPC concentration,WPC processing technique,gastrointestinal pH,and digestion duration had varied effects on the bioaccessibility of 5-MTHF as well as the particle size and potential of WPC-5-MTHF during the digestive process.Among them,the bioaccessibility was 0 and no 5-MTHF was found in any WPC-5-MTHF group throughout the stomach digesting stage.When compared to the 5-MTHF group,the bioaccessibility of the WPC-5-MTHF group improved by 11.1%to 19.61%during the intestinal digestion stage,exhibiting a positive connection trend with the WPC concentration.When compared to the unprocessed treatment group,the bioaccessibility of the ultrasonic,high pressure homogenization groups was reduced by 8.49%,9.52%,and 8.75%,respectively.At an intestinal pH of 7 and a digestion time of 5 hours,the WPC-5-MTHF group displayed the best bioaccessibility,which was twice as high as that of the 5-MTHF group,at 45.17%and 42.32%,respectively.The results of particle size and zeta potential showed that the WPC-5-MTHF group had smaller particle size,larger absolute value of zeta potential,and the structural changes of WPC-5-MTHF would cause changes in digestibility characteristics.Theoretical sup

关 键 词：乳清蛋白 5-甲基四氢叶酸钙 体外消化模拟 生物可及性 微观结构 

分 类 号：TS201.4[轻工技术与工程—食品科学]