罗伊氏粘液乳杆菌JBR3生物学特性分析及保护剂对其活力的影响  

Biological Characteristics Analysis of Limosilactobacillus reuteri JBR3 and the Effect of Protective Agents on Its Viability

作　　者：韩军 王怡 张开屏[3,4] 田建军 HAN Jun;WANG Yi;ZHANG Kaiping;TIAN Jianjun(College of Food Science and Engieering,Inner Mongolia Agricultural University,Hohhot 010018,China;Integrative Research Base of Beef and Lamb Processing Technology Ministry of Agriculture and Rural Affairs of the People's Republic of China,Hohhot 010018,China;Department of Cooking&Food Processing,Inner Mongolia Business and Trade Vocational College,Hohhot 010070,China;Inner Mongolia Alashan Camel Industry Science and Technology Backyard,Alxa League 735400,China)

机构地区：[1]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018 [2]农业农村部生鲜牛羊肉加工技术集成基地,内蒙古呼和浩特010018 [3]内蒙古商贸职业学院餐饮食品系,内蒙古呼和浩特010070 [4]内蒙古阿拉善驼业科技小院,内蒙古阿拉善盟735400

出　　处：《食品工业科技》2025年第3期166-177,共12页Science and Technology of Food Industry

基　　金：阿拉善盟科技计划项目(AMKJ2023-04);内蒙古自治区科技计划(2022YFHH0112);呼和浩特市高校院所协同创新项目(XTCX2023-18)。

摘　　要：为推进罗伊氏粘液乳杆菌IMAUJBR3在肉类发酵剂中的应用,对该菌株进行了全基因组、代谢系统、生物学特性分析,同时采用单因素和响应面试验分析获得乳酸菌冷冻干燥的最佳保护剂比例。结果表明,JBR3的基因组由1个环状染色体和2个环状质粒组成,在代谢系统中注释到gene0325、gene1042等5个产甾醇酯酶(EC3.1.1.13)基因,该酶可以水解生成甾醇和脂肪酸。在益生特性分析中,未注释到生物胺相关基因,但发现了groEL、groES等多个伴侣蛋白基因和冷应激基因gene0733和gene1677,这些基因的存在确保乳酸菌在冷应激期间仍然可以保证菌株的功能特性不被破坏,说明该菌株适合于冷冻干燥。同时,JBR3包含完整的NADH系统、yfeX过氧化物酶、gor谷胱甘肽还原酶、trxA硫氧还蛋白和trxB硫氧还蛋白还原酶,JBR3可能具有较好的抗氧化能力,可以作为发酵剂应用于发酵食品中。JBR3在脱脂乳粉为9.3 g/100 mL、麦芽糊精为2.3 g/100 mL、谷氨酸钠6.8 g/100 mL、海藻糖为10.0 g/100 mL的保护剂复配下冷冻干燥,存活率最高为95.17%±0.57%,活菌数为9.20×10^(10)CFU/g,该菌株具有良好的应用前景。In order to advance the application of Limosilactobacillus reuteri IMAUJBR3 in meat fermenters,the strain was subjected to comprehensive genomic,metabolic and biological analyses.Additionally,one-way and response surface experiments were conducted to identify the optimal ratio of protectants for freeze-drying of lactic acid bacteria.The genome of JBR3 was found to consist of one circular chromosome and two circular plasmids.Five sterol esterase-producing enzyme(EC 3.1.1.13)genes,including gene0325 and gene1042,were identified in the metabolic system,which could be hydrolysed to produce sterols and fatty acids.In the probiotic characterisation,no biogenic amine-related genes were annotated.However,several chaperonin genes,such as groEL and groES,and cold stress genes,gene0733 and gene1677 were found.The presence of these genes ensured that the functional properties of the strain could be maintained during the period of cold stress in lactic acid bacteria.This suggested that the strain was suitable for freeze-drying.In addition,JBR3 contained an intact NADH system,yfeX peroxidase,gor glutathione reductase,trxA thioredoxin and trxB thioredoxin reductase.This suggested that JBR3 might have a superior antioxidant capacity,which could be exploited as a fermentation agent in fermented foods.JBR3 was freeze-dried under a protective agent complex of skimmed milk powder(9.3 g/100 mL),maltodextrin(2.3 g/100 mL),monosodium glutamate(6.8 g/100 mL),and trehalose(10.0 g/100 mL).The survival rate of the strain was 95.17%±0.57%,with a viable bacterial number of 9.20×10^(10) CFU/g.The strain has a promising future in various applications.

关 键 词：罗伊氏粘液乳杆菌 全基因组 冷冻干燥 保护剂 存活率 

分 类 号：TS201.3[轻工技术与工程—食品科学]