张家口艾叶脂溶性成分、抑菌和抗氧化活性分析  

Analysis of Liposoluble Composition,Antibacterial and Antioxidant Activities of Artemisia argyi in Zhangjiakou

作　　者：徐永斌 熊新民 贺友铎 崔佳 拖建刚 赵宏宇 XU Yongbin;XIONG Xinmin;HE Youduo;CUI Jia;TUO Jiangang;ZHAO Hongyu(School of Life Science and Technology,Inner Mongolia University of Science and Technology,Baotou 014010,China;Deosen Biochemical(Ordos)Ltd.,Ordos 014330,China)

机构地区：[1]内蒙古科技大学生命科学与技术学院,内蒙古包头014010 [2]鄂尔多斯市中轩生化股份有限公司,内蒙古鄂尔多斯014330

出　　处：《食品工业科技》2025年第3期288-294,共7页Science and Technology of Food Industry

基　　金：内蒙古自然科学基金(2022MS02009);鄂尔多斯市科技重大专项(2022EEDSKJZDZX007);国家自然科学基金(62261043);内蒙古科技大学基本科研业务费专项资金资助(2023QNJS147)。

摘　　要：张家口地区拥有丰富和优质的艾叶资源,为了有效利用艾叶资源提升其产品价值,采用索氏提取法提取张家口艾叶脂溶性成分,通过GC-MS分析了张家口艾叶脂溶性成分的主要组成,使用滤纸片法测定其对革兰氏阴性菌和革兰氏阳性菌的抑菌活性,考察提取成分对DPPH自由基、羟基自由基和ABTS^(+)自由基的清除能力,分析其抗氧化性能。结果表明:张家口艾叶脂溶性成分中共检测出41种化学成分,其主要化合物类型为脂肪族醇、酯、酮类、酸类以及芳香族类化合物等,相对含量大于1%的成分共有11种,其中4-C-甲基肌醇相对含量高达66.65%。张家口艾叶脂溶性成分对大肠杆菌和金黄色葡萄球菌均具有一定的抑制作用,对金黄色葡萄球菌和大肠杆菌的最小抑菌浓度分别为4.69和9.38μL/mL。张家口艾叶脂溶性成分对DPPH自由基、羟基自由基和ABTS^(+)自由基均具有显著清除效果,IC_(50)分别为12.85、6.32和4.17μL/mL。结果证实张家口艾叶脂溶性成分具有良好的抑菌和抗氧化活性。There are rich and high-quality Artemisia argyi resources in Zhangjiakou region.Soxhlet extraction method was used to extract the liposoluble components of Artemisia argyi in Zhangjiakou which could effectively use Artemisia argyi resources to enhance its product value.The main components of liposoluble components of Zhangjiakou Artemisia argyi were analyzed by GC-MS.The antibacterial activity against Gram-negative bacteria and Gram-positive bacteria was determined by filter paper disk test.The scavenging ability of the extracted components on DPPH radical,hydroxyl radical and ABTS^(+)radical was investigated,and their antioxidant properties were analyzed.The results showed that 41 chemical components were detected in the liposoluble components of Zhangjiakou Artemisia argyi.The main types of compounds were aliphatic alcohols,esters,ketones,acids and aromatic compounds.There were 11 components with relative content greater than 1%,of which 4-C-methyl-myo-linositol comparative content was as high as 66.65%.The liposoluble components of Zhangjiakou Artemisia argyi had certain inhibitory effects on Escherichia coli and Staphylococcus aureus.The minimum inhibitory concentrations of Staphylococcus aureus and Escherichia coli were 4.69 and 9.38μL/mL.The liposoluble components of Zhangjiakou Artemisia argyi had significant scavenging effects on DPPH radical,hydroxyl radical and ABTS^(+)radical,and the IC_(50)was 12.85,6.32 and 4.17μL/mL.The results confirmed that the liposoluble components of Zhangjiakou Artemisia argyi had good antibacterial and antioxidant activities.

关 键 词：张家口艾叶 脂溶性成分 GC-MS分析 抑菌 抗氧化 

分 类 号：R932[医药卫生—生药学]