刺梨复合植物饮料对小鼠急性酒精性肝损伤的保护作用  

Protective Effect of Rosa roxburghii Compound Plant Beverage on Acute Alcohol-induced Liver Injury in Mice

作　　者：漆正方 李东海 孙宜春 杨小生 杨娟 张容榕 王瑜 潘雄 张朝举 李立郎 QI Zhengfang;LI Donghai;SUN Yichun;YANG Xiaosheng;YANG Juan;ZHANG Rongrong;WANG Yu;PAN Xiong;ZHANG Chaoju;LI Lilang(Sinopharm Group Guizhou Healthcare Industry Development Co.,Ltd.,Guiyang 550025,China;College of Pharmacy,Guizhou Medical University,Guiyang 550025,China;Guizhou Natural Products Research Center,Guiyang 550014,China;Sinopharm Group Tongjitang(Guizhou)Pharmaceutical Co.,Ltd.,Guiyang 550025,China)

机构地区：[1]国药集团贵州大健康产业发展有限公司,贵州贵阳550025 [2]贵州医科大学药学院,贵州贵阳550025 [3]贵州省天然产物研究中心,贵州贵阳550014 [4]国药集团同济堂(贵州)制药有限公司,贵州贵阳550025

出　　处：《食品工业科技》2025年第3期361-367,共7页Science and Technology of Food Industry

基　　金：贵州省科技计划项目(黔科合成果[2024]一般087,黔科合支撑[2023]一般077,黔科合成果[2022]一般030);刺梨复合植物饮料开发技术服务(H202213);贵州省教育厅高等学校特色重点实验室建设项目(黔教合KY字[2020]018号)。

摘　　要：本实验以刺梨复合植物饮料(Rosa roxburghii Tratt Compound Plant Beverage,RTCPB)为材料,研究了RTCPB对急性酒精性肝损伤小鼠的保护作用。通过53%的食用酒精灌胃诱导雄性ICR小鼠建立急性肝损伤模型。连续给药21 d,通过测定小鼠肝脏指数、甘油三酯(Triglycerides,TG)的含量、血清谷草转氨酶(Aspartate Aminotransferase,AST)、谷丙转氨酶(Alanine Aminotransferase,ALT)以及肝组织中过氧化氢酶(Catalase,CAT)、超氧化物歧化酶(Superoxide Dismutase,SOD)和谷胱甘肽过氧化物酶(Glutathione Peroxidase,GSHPx)活性、丙二醛(Malondialdehyde,MDA)的含量及肝组织切片病理情况等生理生化指标来评价RTCPB对急性酒精性肝损伤小鼠的保护作用。结果显示,与模型组相比,水飞蓟宾组和RTCPB给药组小鼠肝脏指数显著下降(P<0.05),RTCPB给药组最大降幅为11.78%,RTCPB高剂量组血清中AST、ALT和TG水平分别显著降低50.70%、40.43%、24.64%;肝组织中CAT、SOD、GSH-Px活性分别显著增加33.14%、24.87%、135.04%,MDA含量显著降低29.90%,且肝组织病理切片显示RTCPB给药组小鼠肝损伤情况均有不同程度的改善,尤以RTCPB高剂量组效果最佳。所以,RTCPB对酒精诱导的急性肝损伤具有明显的保护作用。To study the protective effect of Rosa roxburghii Tratt compound plant beverage(RTCPB)against the acute alcoholic liver injury in mice,alcoholic liver injury models were established with 53%alcohol in male ICR mice using oral gavage.The drug was administered continuously for 21 days,the liver index,the content of triglyceride(TG),the activities of aspartate aminotransferase(AST),alanine aminotransferase(ALT),catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)contents were determined to evaluate the potential protective effect of RTCPB on acute alcohol-induced liver injury in mice.Meanwhile,the pathology analysis was performed on liver tissue to determine pathological changes.The results showed that the liver indices of mice in silymarin control and RTCPB groups were significantly decreased(P<0.05)compared with the model group.Moreover,the RTCPB group showed the largest decrease of 11.78%,and the levels of AST,ALT,and TG in RTCPB high-dose group were significantly reduced by 50.70%,40.43%,and 24.64%in serum,respectively.The activities of CAT,SOD and the content of GSH-Px in liver tissues were significantly increased by 33.14%,24.87%,135.04%,and MDA content was significantly reduced by 29.90%.The liver pathological section indicated that the RTCPB groups could alleviate the liver injury,especially for high dose group of RTCPB.Therefore,RTCPB possessed the protective effects on alcohol-induced acute liver injury.

关 键 词：刺梨复合植物饮料(RTCPB) 急性酒精肝损伤 保护作用 抗氧化 

分 类 号：TS201.4[轻工技术与工程—食品科学]