一种增肌及提高行动力功效配方的研究  

Formulation for Muscle Building and Mobility Improvement

作　　者：曹兵兵 刘正冬 葛欢 周佳丽 叶兴旺 祁明媛 刘仁燕 徐懿乔 CAO Bingbing;LIU Zhengdong;GE Huan;ZHOU Jiali;YE Xingwang;QI Mingyuan;LIU Renyan;XU Yiqiao(Hunter Biotechnology,Inc.,Hangzhou 310051,China;Inner Mongolia Mengniu Dairy(Group)Co.,Ltd.,Huhhot 011500,China;Yashili International Group Ltd.,Guangzhou 510335,China)

机构地区：[1]杭州环特生物科技股份有限公司,浙江杭州310051 [2]内蒙古蒙牛乳业(集团)股份有限公司,内蒙古呼和浩特011500 [3]雅士利国际集团有限公司,广东广州510335

出　　处：《食品工业科技》2025年第3期368-375,共8页Science and Technology of Food Industry

摘　　要：以维生素D(Vitamin D,V_(D))、β-羟基-β-甲基丁酸钙(Calcium beta-hydroxy-beta-methylbutyrate,HMBCa)和中长链脂肪酸油(Medium-and long-chain triacylglycerols,MLCT)3种原料按1:350000:200000比例组成组合配方,研究其增肌及提高行动力功效。通过地塞米松诱导在斑马鱼幼鱼中建立肌少症模型,测定其肌肉组织形态、髓鞘的荧光强度及肌肉萎缩相关基因表达水平,评价配方对斑马鱼的增肌作用及机制。通过无水亚硫酸钠诱导斑马鱼疲劳模型,测定其活动量及三磷酸腺苷(Adenosine 5'-triphosphate,ATP)、乳酸和活性氧(Reactive oxygen species,ROS)水平,评价配方对斑马鱼运动行为的改善作用及机制。结果表明:配方能够提高肌少症模型斑马鱼肌纤维质量并改善肌肉组织结构。进一步机制研究发现,配方能够促进髓鞘再生,下调肌肉萎缩相关基因(mstnb、myod1、trim63a和fbxo32)的表达。此外,配方能够提高疲劳模型斑马鱼的活动量。进一步机制研究发现,配方在斑马鱼体内具有增加ATP生成、减少乳酸堆积和促进ROS清除作用。综上,配方具有增肌功效和提高行动力功效。A formulation composed of vitamin D(V_(D)),calciumβ-hydroxy-β-methylbutyrate(HMBCa),and medium-and long-chain triacylglycerols(MLCT)in a precise ratio of 1:350000:200000 was examined for its potential impacts on muscle development and enhancement of mobility.A zebrafish larval model of dexamethasone-induced sarcopenia was used to evaluate the formula's influence on muscle tissue morphology,myelin sheath fluorescence intensity,and the expression of muscle atrophy-related genes.Furthermore,a zebrafish fatigue model induced by sodium sulfite was utilized to assess the formula's effects on mobility,as well as levels of adenosine 5'-triphosphate(ATP),lactate,and reactive oxygen species(ROS)levels,thereby elucidating its impact on zebrafish locomotor behavior and underlying mechanisms.The results indicated that the formulation enhanced muscle fiber quality and improved muscle tissue structure in the zebrafish sarcopenia model.Further detailed studies uncovered that the formulation could stimulate myelin regeneration and suppress the expression of muscle atrophy-related genes(mstnb,myod1,trim63a,and fbxo32).Additionally,the concoction increased the activity level of zebrafish in a fatigue model.More detailed investigations revealed that the formulation could boost ATP production,reduce lactate accumulation,and enhance ROS scavenging in zebrafish.In conclusion,the formula demonstrates significant effects in fostering muscle development and enhancing mobility.

关 键 词：肌少症 维生素D(V_(D)) β-羟基-β-甲基丁酸钙(HMBCa) 中长链脂肪酸油(MLCT) 斑马鱼肌少症模型 斑马鱼疲劳模型 

分 类 号：TS201.4[轻工技术与工程—食品科学]