短时高温对截形叶螨体内糖类物质和游离氨基酸含量变化的影响  

Effects of short-term high temperature on carbohydrate and free amino acid content in Tetranychus truncatus

作　　者：李文亮 张洁 吴霞 罗丽丹 刘磊 王森山 宋丽雯 LI Wen-Liang;ZHANG Jie;WU Xia;LUO Li-Dan;LIU Lei;WANG Sen-Shan;SONG Li-Wen(Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学植物保护学院,甘肃省农作物病虫害生物防治工程实验室,兰州730070

出　　处：《应用昆虫学报》2024年第5期980-988,共9页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金项目(31860503)。

摘　　要：【目的】明确截形叶螨Tetranychus truncatus敏感品系(SS)、抗哒螨灵品系(Py-R)不同螨态体内糖类物质和游离氨基酸含量的变化,探究截形叶螨对短时高温胁迫的适应机理,为叶螨的综合防治提供一定的理论依据。【方法】卵、若螨、雌成螨经高温34、38和42℃分别处理2、4和6 h后,测定叶螨敏感品系与抗哒螨灵品系体内糖类物质和游离氨基酸含量的变化,并分析与高温胁迫的相关性。【结果】截形叶螨敏感品系和抗性品系不同发育阶段经38℃高温处理6h后体内可溶性糖含量达到最大,与对照存在显著差异(P<0.05);抗性品系雌成螨在不同处理温度下,随着胁迫时间的延长,糖原含量均高于敏感品系;不同发育阶段抗性品系体内海藻糖、游离氨基酸含量在34和38℃下均高于敏感品系,42℃高温胁迫2 h,雌成螨抗性品系较敏感品系体内的糖类物质含量上升,存在显著差异(P<0.05)。【结论】截形叶螨体内的糖类物质和游离氨基酸含量与高温胁迫有关,且抗性品系的糖类物质含量在34和38℃高温胁迫下比敏感品系高,说明抗性品系在短时高温胁迫下具有较强的耐受能力,当胁迫温度超过42℃后,抗性品系体内糖类物质含量较敏感品系有所变动,说明极端高温对叶螨有不利影响。本研究结果对田间持续高温综合防控截形叶螨的策略具有一定指导意义。[Aim]To investigate the changes in carbohydrate and free amino acid content in the sensitive strain(SS)and pyridaben resistant strain(Py-R)of Tetranychus truncatus during different developmental stages,and explore the adaptation mechanism of T.truncatus to short-term high temperature stress,providing a theoretical basis for the comprehensive management of T.truncatus.[Methods]Eggs,nymphs and female adults were exposed to 34,38,and 42℃for 2,4,and 6 h,respectively.The content of carbohydrates and free amino acids in the sensitive and pyridaben-resistant T.truncatus strains was measured,and their correlation with high temperature stress analyzed.[Results]The content of soluble sugar and glycogen in the sensitive and resistant strains of T.truncatus initially increased,then decreased following high temperature treatment,showing a significant difference to the control group(P<0.05).Additionally,the contents of trehalose and free amino acids decreased with an increase in temperature and exposure time.[Conclusion]The content of carbohydrates and free amino acids in T.truncatus were influenced by high temperature stress.The pyridaben resistant strain had a higher content of stress-resistant substances compared to the sensitive strain,and demonstrated significant high temperature tolerance,enabling it to withstand short-term high temperature stress.

关 键 词：截形叶螨 高温胁迫 糖类物质 游离氨基酸 

分 类 号：S433.7[农业科学—农业昆虫与害虫防治]