HPLC法测定藤茶中5种黄酮类物质的含量  

Determination of five flavonoids contents in vine tea by HPLC

作　　者：张朝阳 陈娥 马世龙 胡百顺 ZHANG Chaoyang;CHEN E;MA Shilong;HU Baishun(Enshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences,Enshi,Hubei 445000,China)

机构地区：[1]恩施土家族苗族自治州农业科学院,湖北恩施445000

出　　处：《湖南农业大学学报(自然科学版)》2024年第6期54-61,共8页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖北省标准化试点示范项目(HUBS-A-N-10-2023);湖北省地方标准制修订项目(T-X-01-2023224)。

摘　　要：采用C_(18)色谱柱(4.6 mm×250 mm,5μm),以甲醇为流动相A、0.1%磷酸溶液为流动相B,梯度洗脱,(2R,3R)-二氢杨梅素的检测波长为290 nm,(2S,3S)-二氢杨梅素、花旗松素、杨梅苷和杨梅素的检测波长为290 nm(0~<21 min)和255 nm(21~30 min),流速1 mL/min,柱温30℃,进样量10μL,测定5种黄酮类物质的含量。结果表明:(2R,3R)-二氢杨梅素、花旗松素、杨梅苷、杨梅素含量的线性回归方程分别为Y=0.5031X+0.6028,R^(2)=0.9992;Y=0.5904X+0.0720,R^(2)=0.9991;Y=0.4334X-0.0077,R^(2)=0.9995;Y=0.5077X-0.0207,R^(2)=0.9988;线性范围分别为10.60~106.00、2.50~50.00、2.65~53.00、1.06~26.50μg/mL;单波长(290 nm)测定(2R,3R)-二氢杨梅素含量,双波长(290、255 nm)测定花旗松素、杨梅苷、杨梅素含量和(2S,3S)-二氢杨梅素峰面积的方法系统适用性、线性关系、重复性、稳定性和加标回收率良好,精密度高;5种黄酮类物质色谱峰分离度均大于1.5,(2R,3R)-二氢杨梅素色谱峰理论塔板数大于5000,(2S,3S)-二氢杨梅素、花旗松素、杨梅苷、杨梅素色谱峰理论塔板数均大于20000,且单个样品检测时间为30 min;此方法可用于检测藤茶中黄酮类物质的含量。C_(18) column(4.6 mm×250 mm,5μm)with methanol(A)-0.1%-phosphoric acid solution(B)as the mobile phase for gradient elution was used in the HPLC to measure the contents of five flavonoids.The detection wavelength of(2R,3R)-dihydromyricetin was 290 nm,and the detection wavelength of(2S,3S)-dihydromyricetin,taxifolin,myricitrin and myricetin was 290 nm before 21 min and 255 nm from 21 to 30 min.The flow rate was 1 mL/min,the column temperature was maintained at 30℃,and the injection volume was 10μL.The results showed that the linear regression equations of the contents of(2R,3R)-dihydromyricetin,taxifolin,myricitrin and myricetin were Y=0.5031X+0.6028,R^(2)=0.9992,Y=0.5904X+0.0720,R^(2)=0.9991,Y=0.4334X-0.0077,R^(2)=0.9995,Y=0.5077X-0.0207,R^(2)=0.9988,and the linear ranges were 10.6-106,2.50-50.00,2.65-53.00,1.06-26.50μg/mL,respectively.The single-wavelength(290 nm)method for the determination of(2R,3R)-dihydromyricetin content,and the dual-wavelengths(290,255 nm)method for the determination of the contents of taxifolin,myricitrin,myricetin and the peak area of(2S,3S)-dihydromyricetin were superior for system application,linear relationship,repeatability,stability and standard recovery with high precision.The chromatographic peak separations of 5 flavonoids were greater than 1.5,the theoretical plate numbers of(2R,3R)-dihydromyricetin was greater than 5000,the theoretical plate numbers of(2S,3S)-dihydromyricetin,taxifolin,myricitrin,myricetin were greater than 20000,and the detection time of single sample was 30 min.This method could be used for the analysis and detection of flavonoids contents in vine tea.

关 键 词：藤茶 黄酮 高效液相色谱 二氢杨梅素 

分 类 号：S571.1[农业科学—茶叶生产加工]