膳食成分与肠道微生物的孟德尔随机化研究  

Association between dietary components and gut microbiota:a Mendelian randomization study

作　　者：陈海苗 马岩[1] 刘明奇 马珊珊[1] 李军 徐来潮 CHEN Haimiao;MA Yan;LIU Mingqi;MA Shanshan;LI Jun;XU Laichao(Shaoxing Center for Disease Control and Prevention,Shaoxing,Zhejiang 312000,China)

机构地区：[1]绍兴市疾病预防控制中心,浙江绍兴312000

出　　处：《预防医学》2025年第1期73-76,81,共5页China Preventive Medicine Journal

摘　　要：目的采用孟德尔随机化(MR)方法探究碳水化合物、脂肪、蛋白质、糖类膳食成分与119属已知肠道微生物的因果关联。方法膳食成分的全基因组关联研究(GWAS)资料来源于DietGen数据库,肠道微生物的GWAS资料来源于MiBioGen数据库。以与4类膳食成分相关的单核苷酸多态性(SNP)位点为工具变量,以119属已知肠道微生物为研究结局,采用逆方差加权法进行MR分析。采用Cochran Q检验评估异质性,采用MR-Egger回归法检验水平多效性,采用MR-PRESSO检验剔除离群值。采用MAGMA和PLACO分析识别膳食成分与肠道微生物的遗传多效性基因。结果MR分析结果显示,碳水化合物与4属肠道微生物、脂肪与14属肠道微生物、蛋白质与14属肠道微生物、糖类与11属肠道微生物存在因果关联(均P<0.05)。MR-Egger回归法分析结果显示筛选出的SNP不存在水平多效性,MR-PRESSO检验未发现离群值(均P>0.05)。MAGMA和PLACO分析结果显示,74.42%(32/43)的因果关联组存在遗传多效性基因,分别发现了1~10个遗传多效性基因;多对因果关联组存在相同的遗传多效性基因。结论膳食成分与肠道微生物之间具有潜在的遗传关联和因果关联。Objective To explore the causal association between dietary components(carbohydrate,fat,protein,and sug-ar)and 119 genera of known gut microbiota using Mendelian randomization(MR)methods.Methods Genome-wide as-sociation study(GWAS)data for dietary components were collected from the DietGen,while GWAS data for gut micro-biota were collected from the MiBioGen.Single nucleotide polymorphism(SNP)loci associated with the four dietary components were used as instrumental variables,and 119 known gut microbiota genera were used as the outcomes.MR analysis was performed using inverse variance weighted(IVW)method.Heterogeneity was evaluated using Cochran's Q test,horizontal pleiotropy and exclude outliers were tested using MR-Egger regression and MR-PRESSO test.Common genetic pleiotropic genes between dietary components and gut microbiota were identified by MAGMA and PLACO analy-ses.Results The MR analysis revealed causal associations between carbohydrates and 4 gut microbiota genera,fats and 14 genera,proteins and 14 genera,and sugars and 11 genera(all P<0.05).The MR-Egger regression analysis showed no horizontal pleiotropy among the selected SNPs,and the MR-PRESSO test did not identify any outliers(all P>0.05).The MAGMA and PLACO analyses revealed that 74.42%(32/43)of the causal associations had pleiotropic genes,with 1 to 10 pleiotropic genes identified.Multiple causal association groups shared the same pleiotropic genes.Conclusion There are potential genetic and causal associations between dietary components and gut microbiota.

关 键 词：全基因组关联研究 孟德尔随机化 膳食成分 肠道微生物 

分 类 号：R151[医药卫生—营养与食品卫生学]