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作 者:王金贺[1] 王延锋[1] 盛春鸽[1] 于海洋[1] 赵静 王菲 WANG Jinhe;WANG Yanfeng;SHENG Chunge;YU Haiyang;ZHAO Jing;WANG Fei(Mudanjiang Branch,Heilongjiang Academy of Agricultural Sciences,Mudanjiang,Heilongjiang 157041)
机构地区:[1]黑龙江省农业科学院牡丹江分院,黑龙江牡丹江157041
出 处:《北方园艺》2025年第1期75-81,共7页Northern Horticulture
基 金:国家食用菌产业技术体系资助项目(CARS-20)。
摘 要:以板蓝根为试材,木屑培养基为对照,设置4个板蓝根添加量处理,采用袋料栽培的方法,探讨了板蓝根提取液对黑木耳菌丝体生长的影响,研究了板蓝根对黑木耳产量和营养成分的影响及其相关性,以期为中药黑木耳的生产提供参考依据。结果表明:在培养料中适量的添加板蓝根对黑木耳菌丝体生长和子实体生长有显著的促进作用。添加5%板蓝根提取液的黑木耳菌丝的生长速度最快,添加2%板蓝根的产量和生物学效率最高,添加10%板蓝根的培养料显著提高了黑木耳子实体中的总糖和蛋白质含量,降低了黑木耳子实体中5种核苷酸的含量,但对粗脂肪和(R,S)-告依春的含量并未表现出显著的影响。此外,研究还发现黑木耳的产量与蛋白质含量、总糖含量和CMP含量、IMP含量、IMP含量和CMP含量、AMP含量之间存在显著的相关性(P<0.05)。综上,板蓝根可以应用于黑木耳种植,在培养料中适量的添加板蓝根不仅促进黑木耳菌丝体和子实体生长,提高黑木耳子实体中营养活性成分含量,也为中药渣的资源化利用提供新的思路和方法。Taking Isatidis radix as the experimental material,sawdust medium as the control,four Isatidis radix addition treatments were set up.The effect of Isatidis radix extract on the growth of A.heimuer mycelium was studied by bag cultivation method.The effects of Isatidis radix on the yield and nutritional components of A.heimuer and their correlation were studied,in order to provide reference for the production of traditional Chinese medicine black fungus.The results showed that adding appropriate amount of Isatidis radix could significantly promote the growth of mycelium and fruiting body of A.heimuer.The mycelial growth rate of 5%Isatidis radix extract was the highest.Adding 2%Isatidis radix had the highest yield and biological efficiency.The content of total sugar and protein in A.heimuer fruiting body increased significantly were significantly increased by adding 10%Isatidis radix.The content of five nucleotides in the fruiting body of A.heimuer was reduced,but the content of crude fat and(R,S)-goitrin did not show significant effect.In addition,the study also found that there was a significant correlation between the yield of black fungus and protein content,total sugar content and CMP content,IMP content,IMP content and CMP content,AMP content(P<0.05).In summary,Isatidis radix could be applied to the cultivation of A.heimuer.Appropriate addition of Isatidis radix in the culture medium not only promoted the growth of A.heimuer mycelium and fruiting body,improved the nutritional active components in the fruiting body of A.heimuer,but also provided new ideas and methods for the resource utilization of Chinese medicine residue.
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