硒化芹菜素的制备及其抗氧化活性分析  

Synthesis and Antioxidant Activity of Selenized Apigenin

作　　者：高雅文 吴国鹏 韩博 王荣武 娄方明 GAO Yawen;WU Guopeng;HAN Bo;WANG Rongwu;LOU Fangming(College of Chemistry and Environmental Engineering,Hubei Minzu University,Enshi 445000,China)

机构地区：[1]湖北民族大学化学与环境工程学院,湖北恩施445000

出　　处：《食品工业科技》2025年第4期109-116,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金(21964022);湖北民族大学揭榜挂帅项目(JB22004);恩施州科技局项目(D20230087);湖北民族大学研究生教育创新项目(MYK2024002)。

摘　　要：本研究以亚硒酸钠为硒化剂,黄酮类化合物芹菜素(Apigenin,AP)为底物制备硒化芹菜素(Selenated Apigenin,Se-AP)。通过多种分析技术对Se-AP进行结构表征并推测其可能出现的配位结构。由结构表征分析结果可知,硒化修饰后AP在不改变黄酮母环主体结构的基础上形成新的Se-O、C-O-Se化学键,Se元素在Se-AP中均匀分布,并得出Se-AP可能存在的1种配位结构,由原子荧光光谱法测得Se-AP中硒含量约为1.91%±0.11%。设计ABTS^(+)、DPPH、OH自由基清除实验研究硒化前后AP的抗氧化活性。由抗氧化活性实验结果可知,SeAP在400 mg/L时对ABTS^(+)自由基的清除率为93.82%±0.63%,在800 mg/L时对DPPH、OH自由基的清除率分别为39.53%±0.67%、96.54%±0.77%。硒化修饰后AP对DPPH自由基的清除能力略有增强,对ABTS^(+)、OH自由基的清除能力明显提高(IC_(50)分别为53.52±1.85 mg/L、11.33±0.64 mg/L),在800 mg/L时与同浓度下VC清除能力相当。因此说明硒化修饰后AP抗氧化活性提高,生物活性增强,可为硒化黄酮相关食品、药物的研发利用提供新途径。Selenized apigenin(Se-AP)synthesized using sodium selenite employed as a highly reactive selenide reagent and the flavonoid apigenin(AP)as a substrate was investigated in this paper.Followed by a series of analytical techniques were used to characterize structure of Se-AP and extrapolate the possible coordination structures in Se-AP.Se content of Se-AP was measured from the fluorescent spectrophotometry to be about 1.91%±0.11%.Based on the results of structural characterization analysis,there could be concluded that new Se-O and C-O-Se bonds were formed without altering the main structure of the flavonoid backbone in selenized apigenin.The Se elements were evenly distributed in Se-AP,and one possible coordination structure of Se-AP was inferred.In addition,the antioxidant activity of AP and Se-AP was investigated by designing ABTS^(+),DPPH,and OH radical scavenging experiments.The in vitro antioxidant results suggested that Se-AP exhibited a clearance rate of 93.82%±0.63%for ABTS^(+)radicals at a concentration of 400 mg/L,39.53%±0.67%for DPPH radicals and 96.54%±0.77%for OH radicals at a concentration of 800 mg/L and the ability of scavenging DPPH free radicals was slightly enhanced,while the scavenging ability of ABTS^(+)and OH radicals was significantly increased(IC_(50) was 53.52±1.85 mg/L and 11.33±0.64 mg/L),and the scavenging ability was comparable to that of VC at 800 mg/L at the same concentration for Se-AP.Therefore,the present data indicated that selenylation modification of AP could increase the antioxidant activity and enhance the biological activity,which could offer the possibility of the development and utilization of selenized flavonoid-related foods and drugs.

关 键 词：黄酮类化合物 芹菜素 硒化芹菜素 配位结构 抗氧化活性 

分 类 号：R284.3[医药卫生—中药学]