生防菌株贝莱斯芽孢杆菌DJ1液体发酵工艺优化  

Optimization in Liquid Fermentation Process of Bacillus velezensis DJ1

作　　者：姜威[1] 张淑梅[1] 马俊秀 闫更轩 胡基华[1] 吴皓琼[1] JIANG Wei;ZHANG Shumei;MA Junxiu;YAN Gengxuan;HU Jihua;WU Haoqiong(Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin 150010,China)

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010

出　　处：《化学与生物工程》2025年第2期49-54,共6页Chemistry & Bioengineering

基　　金：黑龙江省科学院院长基金项目(YZ2021SW01)。

摘　　要：在单因素实验的基础上,分别采用正交实验、响应面实验优化了贝莱斯芽孢杆菌DJ1的培养基组分和发酵条件。确定最佳培养基组分为:麦芽糖2.5%、酵母粉0.5%、氯化钠1.0%、硫酸锰0.02%;最佳发酵条件为:发酵时间23 h、初始pH值8、发酵温度36℃,在此条件下,OD_(600)值为1.819,与模型预测值基本吻合,发酵液的有效活菌数为2.34×10^(9) CFU·mL^(-1)。On the basis of single-factor experiments,we optimized the medium components and fermentation conditions of Bacillus velezensis DJ1 by orthogonal experiments and response surface methodologies.The optimal medium components are determined as follows:the maltose of 2.5%,the yeast powder of 0.5%,the sodium chloride of 1.0%,and the manganese sulfate of 0.02%.The optimum fermentation conditions are determined as follows:the fermentation time of 23 h,the initial pH value of 8,and the fermentation temperature of 36℃.Under above conditions,the OD_(600) value is 1.819,which is basically consistent with the predicted value of the model,and the amount of effective living bacteria of the fermentation liquid is 2.34×10^(9) CFU·mL^(-1).

关 键 词：贝莱斯芽孢杆菌 响应面法 有效活菌数 发酵工艺 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]