食品中新烟碱类农药免疫快速检测研究进展  

Advances in the Rapid Immunoassay Detection of Neonicotinoid Pesticides in Food

作　　者：寇帅 周玮[1] 朱晓军[1] 匡华[2] 胥传来[2] 郭玲玲 KOU Shuai;ZHOU Wei;ZHU Xiaojun;KUANG Hua;XU Chuanlai;GUO Lingling(Jiangsu Product Quality Testing and Inspection Institute,Nanjing 210042,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江苏省产品质量监督检验研究院,江苏南京210042 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品与生物技术学报》2024年第11期34-43,共10页Journal of Food Science and Biotechnology

基　　金：江苏省市场监督管理局科技计划项目(KJ2022009)。

摘　　要：新烟碱类农药是目前农作物中使用最广泛的神经毒性杀虫剂之一,其残留超标问题一直备受关注。开发新烟碱类农药的高效快速检测技术对于保障食品安全具有重要意义。免疫快速检测凭借技术优势在食品安全监管中得到了广泛应用。作者综述了新烟碱类农药最大残留限量的现行标准,以及常用的新烟碱类农药快速免疫检测技术如酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)技术、免疫层析技术、荧光免疫分析(fluorescence immunoassay,FIA)技术、化学发光免疫分析(chemiluminescence immunoassay,CLIA)技术和仿生ELISA技术的作用原理、特点、研究进展,并评价这些技术的优点与不足。最后对新烟碱类农药在食品安全快速分析检测中面临的挑战和发展前景进行讨论,以期为食品安全免疫快速检测方法的建立提供参考。Neonicotinoid pesticides are one of the most widely used neurotoxic insecticides in crops,and their excessive residue levels have long been a significant concern.Developing efficient,rapid detection technology for neonicotinoid pesticides is crucial for ensuring food safety.Immunoassay-based rapid detection has gained increasing application in food safety monitoring due to its technical advantages.The author primarily reviews the current national standards for maximum residue limits of neonicotinoid pesticides and summarizes the principle,characteristics,and research progress of commonly used rapid immunoassay techniques including enzyme-linked immunosorbent assay(ELISA)technology,immunochromatography assay technology,fluorescence immunoassay(FIA)technology,chemiluminescence immunoassay(CLIA)technology,and biomimetic ELISA technology for their detection.The advantages and limitations of these techniques are also evaluated.Finally,the challenges and prospects of applying rapid analysis and detection of neonicotinoid pesticides in food safety are discussed,with the aim of providing a reference for the development of immunoassay-based rapid detection methods for food safety.

关 键 词：新烟碱类农药 酶联免疫吸附测定 免疫层析 荧光免疫分析 化学发光免疫分析 仿生酶联免疫吸附测定 

分 类 号：F42[经济管理—产业经济]