木棉不同颜色花瓣黄酮类物质代谢组学分析  

Metabolomic Analysis on Flavonoids in Bombax ceiba Petals with Different Colors

作　　者：杨会肖[1] 桑贤东 崔雯珺 朱报著[1] 廖焕琴 张卫华[1] 廖柏勇 徐放[1] 徐斌[1] 杨晓慧[1] Yang Huixiao;Sang Xiandong;Cui Wenjun;Zhu Baozhu;Liao Huanqin;Zhang Weihua;Liao Boyong;Xu Fang;Xu Bin;Yang Xiaohui(Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangdong Academy of Forestry,Guangzhou,Guangdong 510520,China;College of Horticulture and Landscape Architecture,Zhongkai University of Agricultural and Engineering,Guangzhou,Guangdong 510230,China;College of Agriculture and Biology,Zhongkai University of Agricultural and Engineering,Guangzhou,Guangdong 510230,China)

机构地区：[1]广东省森林培育与保护利用重点实验室,广东省林业科学研究院,广东广州510520 [2]仲恺农业工程学院园艺园林学院,广东广州510230 [3]仲恺农业工程学院农业与生物学院,广东广州510230

出　　处：《广西林业科学》2024年第6期793-800,共8页Guangxi Forestry Science

基　　金：广东省自然科学基金项目(面上项目)(2214050006239)。

摘　　要：为揭示木棉(Bombax ceiba)花颜色差异的代谢机理,为后续木棉新品种创制研究提供理论依据,以深红色、红色、橙红色、橙黄色和黄色木棉花花瓣为试验材料,采用高效液相色谱-串联质谱法(HPLC-MS/MS),对5种木棉花花瓣进行代谢组学测定,分析花瓣黄酮类物质组成和含量。结果表明,共鉴定出6类137种黄酮类代谢物,分别为黄酮和黄酮醇(79种)、黄烷酮(19种)、花青素(14种)、异黄酮(12种)、查耳酮和二氢查耳酮(7种)及类黄酮(6种)。黄酮和黄酮醇代谢物在5种颜色花瓣中含量均最高,占比均超过65%;其次为黄烷酮和花青素代谢物。花青素代谢物分析显示,花青素苷、矢车菊素3-O-芸香糖苷、矢车菊素-3-半乳糖苷和矢车菊素3-O-葡萄糖苷在木棉深红色花瓣中含量均较高。KEGG分析显示,5种颜色花瓣的差异代谢物均显著富集在花青素合成通路上。影响木棉花花瓣呈红色的主要代谢物质是花青素,呈现黄色可能为高含量的黄酮和黄酮醇代谢物及低含量的花青素类物质。In order to reveal metabolic mechanisms underlying flower color differences in Bombax ceiba and provide theoretical basis for development of new cultivars,B.ceiba petals with five different colors including dark red,red,orange red,orange yellow and yellow were used as experimental materials.Metabolomic analysis was performed on B.ceiba petals using high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)technology to analyze compositions and contents of flavonoids.Results showed that total of 137 flavonoids metabolites from six classes were identified,including flavones and flavonols(79),flavanones(19),anthocyanins(14),isoflavonoids(12),chalcones and dihydrochalcones(7)and flavonoids(6).Contents of flavonoids and flavonols metabolites were the highest in all five petals,accounting for more than 65%,followed by flavanones and anthocyanins metabolites.Anthocyanin metabolie analysis revealed that cyanin chloride,keracyanin chloride,idaein chloride and cyanidin 3-O-glucoside were the highest in dark red petals of B.ceiba.KEGG analysis indicated that differential metabolites in all five petals were significantly enriched in anthocyanin synthesis pathway of anthocyanin.Primary metabolites responsible for red coloration of B.ceiba petals were anthocyanins,while yellow coloration might be associated with high levels of flavones and flavonols and low levels of anthocyanins.

关 键 词：花色 代谢组 花青素 木棉 

分 类 号：S685.99[农业科学—观赏园艺]