一株瘤胃高产菌体蛋白酵母菌的筛选鉴定及发酵条件优化  

Screening and identification of a high-producing soluble protein yeast strain from rumen and optimization of fermentation conditions

作　　者：徐凯 池芝莉 柴迎楠 全宏冬 李鸿雁 叶陆仟 刘占英 XU Kai;CHI Zhi-li;CHAI Ying-nan;QUAN Hong-dong;LI Hong-yan;YE Lu-qian;LIU Zhan-ying

机构地区：[1]内蒙古自治区发酵产业节能减排工程技术研究中心,内蒙古呼和浩特010051 [2]生物发酵绿色制造内蒙古自治区工程研究中心,内蒙古呼和浩特010051 [3]内蒙古自治区生物发酵产业专业化技术研发与中试公共服务平台,内蒙古呼和浩特010051 [4]内蒙古工业大学化工学院,内蒙古呼和浩特010051 [5]赤峰瑞阳化工有限公司,内蒙古赤峰024000

出　　处：《饲料研究》2024年第24期101-106,共6页Feed Research

基　　金：内蒙古杰出青年基金项目(项目编号:2022JQ10);内蒙古自治区“草原英才”工程产业创新人才团队项目(项目编号:2022JQ10);内蒙古自治区多元醇化工新材料企业重点实验室开放基金(项目编号:ZZQzdsys005)。

摘　　要：试验旨在从牛瘤胃中分离筛选出高产菌体蛋白且耐铵盐的酵母菌。试验共筛选出5株酵母菌,分别为CZL-1、CZL-2、CZL-3、CZL-4、CZL-5,对5株酵母菌的最适温度、pH值、菌体蛋白含量及铵盐耐受性进行测定。结果显示,单因素试验优化温度为37℃、pH值5.0、发酵周期48 h。CZL-3的生物量达最优且酵母菌菌体中蛋白含量从初始47.79%提升至68.10%,提高了42.50%(P<0.05)。通过形态学观察,生理生化鉴定和18S rDNA序列分析CZL-3菌株为热带假丝酵母(Candida tropicalis)。NH_(4)^(+)浓度超过0.6 mol/L时,5株酵母菌的菌体蛋白含量显著降低(P<0.05)。研究表明,试验分离菌株具有高产菌体蛋白及耐铵盐的特性,有望作为发酵生产菌体蛋白饲料中的优质菌种。The experiment aimed to isolate and screen yeast strains that are high in microbial protein production and tolerant to ammonium salts from the rumen of cattle.A total of five yeast strains were screened,named CZL-1,CZL-2,CZL-3,CZL-4,and CZL-5.The optimal temperature,pH value,microbial protein content,and ammonium salt tolerance of the five yeast strains were determined.The results showed that the optimal temperature of single factor test was 37℃,pH value was 5.0,and the fermentation period was 48 h.The biomass of CZL-3 reached the optimal level,and the protein content of yeast was increased by 42.50%from the initial 47.79%to 68.10%(P<0.05).Through morphological observation,physiological and biochemical identification,and 18S rDNA sequence analysis,strain CZL-3 was identified as Candida tropicalis.When the NH_(4)^(+) concentration exceeded 0.6 mol/L,the microbial protein content of the five yeast strains significantly decreased(P<0.05).The study indicates that the isolates have high yield of bacterial protein and resistance to ammonium salt,and are expected to be used as high quality strains in the fermentation production of bacterial protein feed.

关 键 词：瘤胃 酵母菌 菌体蛋白 发酵条件优化 

分 类 号：S816.7[农业科学—饲料科学]