牡丹花残渣提取物制备工艺优化及其抗氧化活性研究  

Optimization of the Extraction Process of Residue Extract from the Flowers of Paeonia suffruticosa and Study on Its Antioxidant Activity

作　　者：左亚锋 蒋祥明 司益榕 吴德玲 张伟 栗进才 孟祥松 汤建 徐秀泉 Zuo Yafeng;Jiang Xiangming;Si Yirong;Wu Deling;Zhang Wei;Li Jincai;Meng Xiangsong;Tang Jian;Xu Xiuquan(School of Chinese Medicine,Bozhou University,Bozhou 236800,China;Key Lab.of Chinese Medicine Materials Research of Anhui Higher Education Institutes,Bozhou 236800,China;Anhui Province Key Lab.of Traditional Chinese Medicine Decoction Pieces of New Manufacturing Technology,School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China)

机构地区：[1]亳州学院中药学院,安徽亳州236800 [2]中药原料产品研发安徽普通高校重点实验室,安徽亳州236800 [3]安徽中医药大学药学院中药饮片制造新技术安徽省重点实验室,安徽合肥230012

出　　处：《中国野生植物资源》2024年第12期35-42,共8页Chinese Wild Plant Resources

基　　金：安徽省高校优秀青年教师培育项目(YQYB2023064);安徽高校协同创新项目(GXXT-2023-073);国家重点研发计划项目(2023YFC3504202);国家级大学生创新创业训练计划项目(202412926020);安徽省大学生创新创业训练计划项目(S202412926066)。

摘　　要：目的:优化超声辅助乙醇溶剂法提取牡丹花残渣提取物中总黄酮和总多酚的工艺条件,并对其抗氧化活性进行研究。方法:以牡丹花残渣提取物中总黄酮和总多酚得率为评价指标,采用单因素和响应面法优化其提取工艺,并通过DPPH法、ABTS法和Fe3+还原能力法评价其抗氧化活性。结果:牡丹花残渣提取物中总黄酮和总多酚提取最佳工艺条件为乙醇浓度70%、液料比42mL/g、超声温度41℃。此条件下牡丹花残渣提取物中总黄酮和总多酚提取率分别为0.72%和2.51%。抗氧化活性实验表明,牡丹花残渣提取物在1.0mg/mL条件下对DPPH、ABTS自由基清除能力和Fe^(3+)还原力吸光度值为73.96%、100.00%、1.204。结论:牡丹花残渣提取物中活性成分具有显著抗氧化活性,可为牡丹花残渣的开发利用提供理论参考。Objective:To optimize the process conditions for extracting total flavonoids and total polyphenols from residue extract of the flowers of Paeonia suffruticosa by ultrasound-assisted ethanol solvent method and study their antioxidant activities.Methods:With the extraction yields of total flavonoids and total polyphenols in the residue extract from the flowers of P.suffruticosa as the evaluation indexes,single-factor experiments and response surface methodology were used to optimize the extraction process.The antioxidant activity was evaluated by DPPH radical scavenging activity,ABTS radical scavenging activity and Fe^(3+) reducing activity.Results:The optimal process conditions for the extraction of total flavonoids and total polyphenols from residue extract of the flowers of P.suffruticosa were ethanol concentration of 70%,liquid-solid ratio of 42 mL/g,and extraction temperature of 41℃.Under these conditions,the extraction yields of total flavonoids and total polyphenols were 0.72% and 2.51%,respectively.The results of antioxidant activity experiments showed that at a concentration of 1.0 mg/mL,the DPPH radical scavenging ability,ABTS radical scavenging ability and Fe3+reducing power of the residue extract from the flowers of P.suffruticosa were 73.96%,100.00%,and 1.204,respectively.Conclusion:Residue extract from the flowers of P.suffruticosa had high content of active ingredients and antioxidant activity.It could provide theoretical reference for the development and utilization of residue from the flowers of P.suffruticosa.

关 键 词：牡丹花残渣 制备工艺 总黄酮 总多酚 抗氧化活性 

分 类 号：Q946[生物学—植物学]