基于糖苷水解酶家族基因的双歧杆菌比较基因组学分析及其在两歧双歧杆菌分离中的应用  

Comparative Genomic Analysis of Bifidobacterium Based on Glycoside Hydrolase Family Genes and Its Application in the Isolation of B.bifidum

作　　者：李岩 于航 陈思远 姜楠 赵鹏昊 孟祥晨[1] LIYan;YU Hang;CHEN Siyuan;JIANG Nan;ZHAO Penghao;MENG Xiangchen(Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨150030

出　　处：《食品科学》2025年第5期114-123,共10页Food Science

基　　金：国家自然科学基金面上项目(32272291)。

摘　　要：本研究旨在比较分析双歧杆菌共有与特有的糖苷水解酶家族基因,在此基础上建立一种从婴儿粪便样本中快速分离目标双歧杆菌的方法。首先比较分析短双歧杆菌(Bifidobacterium breve)、长双歧杆菌长亚种(B. longum subsp. longum)、两歧双歧杆菌(B. bifidum)和长双歧杆菌婴儿亚种(B. longum subsp. infantis)4种双歧杆菌的糖苷水解酶家族基因,发现这些菌株在糖苷水解酶家族基因上存在共有及特有模式,可作为双歧杆菌分离和鉴定的分子标记。基于菌落聚合酶链式反应和核酸电泳技术,成功开发出一种快速分离和初步鉴定上述双歧杆菌的方法,并将其应用于B. bifidum的分离,该方法不仅提高了分离效率,而且降低了成本。本研究结果有利于加深对双歧杆菌属细菌多样性的认识,可为未来益生菌的研发奠定基础。This study aimed to comparatively analyze the common and unique glycoside hydrolase(GH)family genes in Bifidobacterium.On this basis,a rapid method for isolating target Bifidobacterium strains from infant fecal samples was developed.The GH family genes of four bifidobacterial taxa,including B.breve,B.longum subsp.longum,B.bifidum and B.longum subsp.infantis,were compared,revealing that there were common and unique patterns in the GH family genes among these species,which could serve as molecular markers for the isolation and identification of Bifidobacterium strains.Based on colony polymerase chain reaction(PCR)and nucleic acid electrophoresis,a rapid method for the isolation and identification of the aforementioned bifidobacterial strains were successfully developed and applied to the isolation of B.bifidum.This method is characterized by not only high efficiency of isolation but also low costs.The results of this study contribute to a better understanding of the diversity of Bifidobacterium,thereby laying a foundation for future probiotic research and development.

关 键 词：比较基因组学 糖苷水解酶 双歧杆菌属 分离鉴定 

分 类 号：Q93[生物学—微生物学]