A novel depolymerase specifically degrades the K62-type capsular polysaccharide of Klebsiella pneumoniae  

作　　者：Yuqing Pan Huagen Chen Ruijing Ma Yongqin Wu Heyuan Lun Aixi Wang Kai He Jingran Yu Ping He 

机构地区：[1]Department of Immunology and Microbiology,Shanghai Jiao Tong University,School of Medicine,Shanghai 200025,China [2]Shanghai Reinovax Biologics Co.,Ltd.,Pudong New District,Shanghai 201210,China

出　　处：《One Health Advances》2024年第1期317-326,共10页全健康进展(英文)

基　　金：funded by the the National Key Research and development Project of China(grant no.2022YFA1304300);National Natural Science Foundation of China(grant no.81971896).

摘　　要：Carbapenem-resistant Klebsiella pneumoniae(CRKP)is emerging as an imminent threat to worldwide public health because of its high level of antimicrobial resistance,which can result in severe and challenging-to-treat infections.The capsular polysaccharide(CPS)of bacteria is well acknowledged as a crucial virulence factor that shields K.pneumoniae from the host’s innate immune system.Polysaccharide depolymerase,encoded by bacteriophages,can hydrolyze the CPS of K.pneumoniae and may be a promising approach for treating K.pneumoniae infections.In this study,we identified a novel K62-type capsule depolymerase(K62-Dpo30)from the K.pneumoniae phage SH-KP2492.We dem-onstrated that the K62-Dpo30 depolymerase could specifically degrade the CPS of K62-type K.pneumoniae strains and promote the susceptibility of K62-type K.pneumoniae strains to serum and neutrophil killing.Furthermore,our findings highlight the potential of the K62-Dpo30 depolymerase as a reliable K.pneumoniae capsular typing tool.

关 键 词：Klebsiella pneumoniae BACTERIOPHAGE Capsule depolymerase 

分 类 号：R57[医药卫生—消化系统]