Continuous immunosuppression is required for suppressing immune responses to xenografts in non‑human primate brains  

作　　者：Su Feng Ting Zhang Zhengxiao He Wenchang Zhang Yingying Chen Chunmei Yue Naihe Jing 

机构地区：[1]Guangzhou National Laboratory,Guangzhou 510005,China [2]Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200080,China [3]National Clinical Research Center for Eye Disease,Shanghai 200080,China [4]Shanghai Key Laboratory of Ocular Fundus Diseases,Shanghai 200080,China [5]Suzhou Yuanzhan Biotechs,Suzhou 215000,China

出　　处：《Cell Regeneration》2024年第1期305-314,共10页细胞再生(英文)

基　　金：National Key Basic Research and Development Program of China(2019YFA0801402,2018YFA0107200,2018YFA0801402,2018YFA0800100);Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16020501,XDA16020404);National Natural Science Foundation of China(32130030,31900454).

摘　　要：Continuous immunosuppression has been widely used in xenografts into non-human primate brains.However,how immune responses change after transplantation in host brains under continuous immunosuppressive adminis-tration and whether immunosuppression can be withdrawn to mitigate side effects remain unclear.Human induced neural stem/progenitor cells(iNPCs)have shown long-term survival and efficient neuronal differentiation in primate brains.Here,we evaluate the immune responses in primate brains triggered by human grafts.The results show that the immune responses,including the evident activation of microglia and the strong infiltration of lymphocytes(both T-and B-cells),are caused by xenografts at 4 months post transplantation(p.t.),but significantly reduced at 8 months p.t.under continuous administration of immunosuppressant Cyclosporin A.However,early immuno-suppressant withdrawal at 5 months p.t.results in severe immune responses at 10 months p.t.These results suggest that continuous long-term immunosuppression is required for suppressing immune responses to xenografts in pri-mate brains.

关 键 词：Cell transplantation XENOGRAFT Immune response Non-human primate brain Human induced neural stem/progenitor cells(iNPCs) 

分 类 号：R73[医药卫生—肿瘤]