A multi-omics analysis of viral nucleic acid poly(I:C)responses to mammalian testicular stimulation  

作　　者：Donghui Yang Wenping Wu Qizhong Lu Yaling Mou Wenbo Chen Shicheng Wan Mengfei Zhang Congliang Wang Xiaomin Du Na Li Jinlian Hua 

机构地区：[1]Present Address:College of Veterinary Medicine,Shaanxi Centre of Stem Cells Engineering&Technology,Northwest A&F University,No.3rd,Taicheng Road,Yangling 712100,Shaanxi,China [2]State Key Laboratory of Biotherapy and Cancer Center,West China Hospital,Sichuan University,Chengdu 610041,China [3]Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats,College of Life Sciences,Yulin University,Yulin 719000,Shaanxi,China

出　　处：《Stress Biology》2024年第1期680-691,共12页逆境生物学(英文)

基　　金：supported by the National Natural Science Foundation of China(32072806,20230978);the National Key Research and Development Program of China(2022YFD1302201,2023YFF1000904,2023ZD0404303);Xinjiang Ugur Autonouous Region Scientific Research and Innovation Platform Construction Project“State Key Laboratory of Genetic Improvement and Germplasm Innovation of Grass-fed Livestock Coconstruction by the Province and Ministry”,Key Technologies Demonstration of Animal Husbandry in Shaanxi Province(20221086,20230978);Inner Mongolia Autonomous Region posted leaders(2022JBGS0025).

摘　　要：The male reproductive system has a standard immune response regulatory mechanism,However,a variety of external stimuli,including viruses,bacteria,heat,and medications can damage the testicles and cause orchitis and epididymitis.It has been shown that various RNA viruses are more likely to infect the testis than DNA viruses,inducing orchitis and impairing testicular function.It was found that local injection of the viral RNA analog poly(I:C)into the testes markedly disrupted the structure of the seminiferous tubules,accompanied by apoptosis and inflammation.Poly(I:C)mainly inhibited the expression of testosterone synthesis-associated proteins,STAR and MGARP,and affected the synthesis and metabolism of amino acids and lipids in the testis.This led to the disruption of the metabolite levels in the testis of mice,thus affecting the normal spermatogenesis process.The present study analyzed the acute inflammatory response of the testis to viral infection using a multi-omics approach.It provides insights into how RNA virus infection impairs testicular function and offers a theoretical basis for future studies on immune homeostasis and responses under stress conditions in male reproduction.

关 键 词：ORCHITIS Poly(I:C) Proteomic METABOLOMIC Homeostasis 

分 类 号：O62[理学—有机化学]