Exogenous Beta-guanidinopropionic acid administration enhances electromyostimulation-induced mitochondrial biogenesis in rat skeletal muscle  

作　　者：Naoki Fukao Junya Takegaki Ryo Takagi Koki Okumura Satoshi Fujita 

机构地区：[1]Graduate School of Sport and Health Science,Ritsumeikan University,Kusatsu,Japan [2]Research Organization of Science and Technology,Ritsumeikan University,Kusatsu,Shiga,Japan [3]Graduate School of Agricultural Science,Kobe University,Kobe,Hyogo,Japan [4]Ritsumeikan Global Innovation Research Organization,Ritsumeikan University,Kusatsu,Japan [5]School of Nursing and Rehabilitation Sciences,Showa University,Yokohama,Japan [6]Faculty of Sport and Health Science,Ritsumeikan University,Kusatsu,Shiga,Japan

出　　处：《Translational Exercise Biomedicine》2024年第3期295-304,共10页运动转化生物医学(英文)

基　　金：supported by JSPS KAKENHI Grant Number JP21KK0177 to SF.

摘　　要：Objectives Exercise training induces several skeletal muscle adaptations.Beta-guanidinopropionic acid(β-GPA)is a creatine analog that simulates the effect of exercise to induce mitochondrial biogenesis.However,the effects ofβ-GPA on resistance training adaptation,such as muscle hypertrophy and mitochondrial biogenesis,are unclear.Therefore,using a resistance exercise model in rats,the present study was designed to investigate the effects ofβ-GPA administration on resistance training adaptations.Methods This study was approved by the Ethics Committee for Animal Experiments at Ritsumeikan University(approval number:BKC2022-009).Male Sprague Dawley rats were randomly divided into placebo orβ-GPA groups.β-GPA(1000 mg/kg)was orally administered once daily,starting seven days before the initiation of electromyostimulation as a model for resistance exercise,and continued throughout the training period.Electromyostimulation was applied to the right gastrocnemius muscle via electrical stimulation every other day for a total of 12 sessions Results Peroxisome proliferators-activated receptor-γco-activator-1α,a regulator of mitochondrial biogenesis,was significantly increased by the combination of training andβ-GPA compared to the training leg(p<0.05).Protein expression of Total OXPHOS,a marker of mitochondrial content,was significantly increased by the combination of training andβ-GPA compared to the training leg(p<0.05).β-GPA intake reduced muscle mass(main effect ofβ-GPA,p<0.05)and was associated with muscle protein breakdown-related Fbx32 and LC3-II protein expression levels but did not counteract the increase in muscle mass caused by resistance training.Conclusions Administration of exogenousβ-GPA enhanced resistance training-induced mitochondrial biogenesis.Moreover,β-GPA still permitted resistance electromyostimulation-induced muscle mass gains,but that effect was attenuated as compared to placebo.

关 键 词：PGC-1Α AMPK OXPHOS CALCINEURIN muscle proteolysis 

分 类 号：TG1[金属学及工艺—金属学]